Target of this antibody:
Alternative names of antibody target:
Damage-regulated autophagy modulator
Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been sμggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and sμggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
DRAM antibody was raised against a 16 amino acid peptide from near the amino terminus of human DRAM.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.
Affinity chromatography purified via peptide column.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry, ELISA
Western Blot: 0.5-2 μg/ml, Immunohistochemistry: 2.5 μg/ml, ELISA. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.