Nano Fluorescent Size Standard Kit, Flow Cytometry Grade, Yellow, 1E6/mL, 0.1-0.3µm, 0.4-0.6µm, 0.7- 0.9µm, &1.0-1.9µm, 4x5mL

• Catalog number
  NFPPS-52-4K
• Price
  Please ask
• Size
  4x 5 mL

• Description
  Nano Fluorescent Size Standard Kit, Flow Cytometry Grade, Yellow, 1E6/mL, 0.1-0.3µm, 0.4-0.6µm, 0.7- 0.9µm, &1.0-1.9µm, 4x5mL are a used in cell biology assays such as cell counting, cell separation, flow cytometry (FC), calibration of flow cytometer machines and so on. Guaranteed to meet the size specification and produced from high quality materials, these microparticles will be a handy tool in your lab work.
• Notes
  For research use only.
• Additional description
  FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

• Gene target
  Nano   Size   Kit   Grade   1E6   1-0   3µm   4-0   6µm   9µm   0-1   9µm   4x5mL  
• Gene symbol
  IGKV1OR2-0, SNORD112, H1-0, IGHV7-4-1, TRUND-NNN4-1, TRQ-CTG1-4, TRW-CCA4-1, TRK-TTT4-1, TRV-AAC4-1, TRP-TGG4-1
• Short name
  Nano Size Standard Kit, Grade, , 1E6/ , 1- 3µm, 4- 6µm, 7- 9µm, &1 -1 9µm, 4x5mL
• Technique
  Flow Cytometry, Standard, Flow, standards
• Label
  fluorescent
• Alternative name
  Nano Fluorescent Size reference reagent, Flow Cytometry Grade, Yellow, 1E6/milliliter, 0.1-0.3µm, 0.4-0.6µm, 0.7- 0.9µm, &1.0-1.9µm, 4x5mL
• Alternative technique
  standards, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:5766
• Gene
  IGKV1OR2-0
• Long gene name
  immunoglobulin kappa variable 1/OR2-0 (non-functional)
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR2-0
• Synonyms
  • IGKV1/OR2-0
• GenBank acession
  • Y08392
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28867
• Classification
  • Immunoglobulin kappa (IGK) orphons

Gene info

• Identity
  HGNC:32777
• Gene
  SNORD112
• Long gene name
  small nucleolar RNA, C/D box 112
• Synonyms
  • 14q(0)
• Locus
  14q32.31
• Discovery year
  2006-05-08
• Entrez gene record
  692215
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003080
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:4714
• Gene
  H1-0
• Long gene name
  H1.0 linker histone
• Synonyms gene
  • H1FV
  • H1F0
• Synonyms gene name
  • H1 histone family, member 0
  • H1 histone family member 0
• Synonyms
  • H1.0
• Synonyms name
  • H1.0, H1(0), H1-0
• GenBank acession
  • X03473
• Locus
  22q13.1
• Discovery year
  1988-07-20
• Entrez gene record
  3005
• Pubmed identfication
  • 3084796
• RefSeq identity
  • NM_005318
• Classification
  • H1 histones
• VEGA ID
  OTTHUMG00000150659

Gene info

• Identity
  HGNC:5665
• Gene
  IGHV7-4-1
• Long gene name
  immunoglobulin heavy variable 7-4-1
• GenBank acession
  • L10057
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  57289
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191141

Gene info

• Identity
  HGNC:50816
• Gene
  TRUND-NNN4-1
• Long gene name
  tRNA-undetermined (NNN) 4-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 4-1
• Synonyms
  • tRNA-Und-NNN-4-1
• GenBank acession
  • HG984093
• Locus
  1p36.23
• Discovery year
  2014-06-20
• Entrez gene record
  103482530
• Pubmed identfication
  • 18984615
• Classification
  • Cytoplasmic transfer RNA pseudogenes

Gene info

• Identity
  HGNC:34884
• Gene
  TRQ-CTG1-4
• Long gene name
  tRNA-Gln (anticodon CTG) 1-4
• Synonyms gene
  • TRNAQ24
  • TRQ-CTG1-1
• Synonyms gene name
  • transfer RNA glutamine 24 (anticodon CUG)
  • transfer RNA-Gln (CTG) 1-1
  • transfer RNA-Gln (CTG) 1-4
• Synonyms
  • tRNA-Gln-CTG-1-4
• GenBank acession
  • HG983716
• Locus
  15q22.31
• Discovery year
  2008-08-29
• Entrez gene record
  100189317
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34733
• Gene
  TRW-CCA4-1
• Long gene name
  tRNA-Trp (anticodon CCA) 4-1
• Synonyms gene
  • TRNAW5
• Synonyms gene name
  • transfer RNA tryptophan 5 (anticodon CCA)
  • transfer RNA-Trp (CCA) 4-1
• Synonyms
  • tRNA-Trp-CCA-4-1
• GenBank acession
  • HG984069
• Locus
  12q23.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189171
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34612
• Gene
  TRK-TTT4-1
• Long gene name
  tRNA-Lys (anticodon TTT) 4-1
• Synonyms gene
  • TRNAK8
• Synonyms gene name
  • transfer RNA lysine 8 (anticodon UUU)
  • transfer RNA-Lys (TTT) 4-1
• Synonyms
  • tRNA-Lys-TTT-4-1
• GenBank acession
  • HG983936
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189053
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34723
• Gene
  TRV-AAC4-1
• Long gene name
  tRNA-Val (anticodon AAC) 4-1
• Synonyms gene
  • TRNAV17
• Synonyms gene name
  • transfer RNA valine 17 (anticodon AAC)
  • transfer RNA-Val (AAC) 4-1
• Synonyms
  • tRNA-Val-AAC-4-1
• GenBank acession
  • HG984107
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189161
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35056
• Gene
  TRP-TGG4-1
• Long gene name
  tRNA-Pro (anticodon TGG) 4-1
• Synonyms gene
  • TRNAP26P
• Synonyms gene name
  • transfer RNA proline 26 (anticodon UGG) pseudogene
  • transfer RNA-Pro (TGG) 4-1
• Synonyms
  • tRNA-Pro-TGG-4-1
• GenBank acession
  • HG984002
• Locus
  16p13.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189483
• RefSeq identity
  • NG_008541
• Classification
  • Cytoplasmic transfer RNA pseudogenes

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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