Rubella, Strain HPV-77 (German Measles) (FITC)
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Catalog number
MBS613767
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Price
Please ask
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Size
1 mL
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Products_type
Antibody
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Products_short_name
[Rubella, Strain HPV-77]
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Products_name_syn
[Anti -Rubella, Strain HPV-77 (German Measles) (FITC)]
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Clonality
Polyclonal
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Specificity
Purified virions. Uninfected Cell Reactivity: Negative vs. Vero cells by by indirect immunofluorescence
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Purity
Highly Purified Purified IgG fraction to a purity of > 95%.
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Form
Supplied as a liquid in PBS, pH 7.2, BSA (10mg/ml), 0.1% sodium azide. Conjugated to FITC.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
Immunofluorescence (IF)
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
CSTF3, OTUD6B, SNORD10, RNU6-77P
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Short name
Rubella, Strain HPV-77 (German Measles) (FITC)
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Technique
FITC, Fluorescein
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Host
Goat
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Isotype
IgG
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Label
FITC
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Alternative name
Rubella, Strain cervical cancer virus-77 (German Measles) (fluorecein)
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Alternative technique
fluorescine
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Virus
measles, rubella, strain
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Gene info
Gene info
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Identity
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Gene
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Long gene name
OTU deubiquitinase 6B
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Synonyms gene name
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Synonyms
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Locus
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Discovery year
2005-09-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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