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HSP27 ELISA Kit

HSP27 ELISA Kit is available 2 times from Stressmark kits labs

SKT-109-480 | HSP27 ELISA Kit size: 5 x 96 well | 2,674.91 USD

Catalog number SKT-109-480
Price 2,674.91 USD
Size 5 x 96 well
Net weight (g) 2500
1. Gene info
Identity5246
GeneHSPB1
Synonyms gene name
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
GenBank acession
Locus7q11.23
Discovery year1991-07-09
Pubmed identfication
Havana BLAST/BLATOTTHUMG00000023228
2. Gene info
Identity10520
GeneSAFB
Synonyms
GenBank acession
Locus19p13.3
Discovery year1998-01-27
Pubmed identfication
Havana BLAST/BLATOTTHUMG00000180502
MeSH Data
Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360

SKT-109-96 | HSP27 ELISA Kit size: 96 well | 671.48 USD

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Stock availability In Stock
Kit's description Colorimetric detection of HSP27
Protein target HSP27
Scientific context HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermotolerance invivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6). Looking for more information on HSP27? Visit our new HSP27 Scientific Resource Guide at more.
Primary research area Cancer, Heat Shock
Category Assay ELISA Kits
Other name 28kDa heat shock protein ELISA Kit, CMT2F ELISA Kit, HSP25 ELISA Kit, HSP27 ELISA Kit, HSP28 ELISA Kit, HSPB1 ELISA Kit, SRP27 ELISA Kit
Brand name StressXpress®
Detection system Colorimetric Assay
Assay format Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Main intended usage ELISA kit used to quantitate HSP27 concentration in samples.
Brief protocol 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Kit contents Anti-Hsp27 Immunoassay Plate, 5X Hsp27 Extraction Reagent, Recombinant Hsp27 Standard, Standard and Sample Diluent, 10X Wash Buffer Concentrate, Anti-Hsp27 Biotinylated Antibody Concentrate, Anti-Hsp27 Biotinylated Antibody Diluent, Streptavidin: HRP Concentrate, Streptavidin: HRP Diluent, TMB Substrate, Stop Solution
Assay precision Contact us
Assay cross-reactivity Human
Samples to be used with this kit Serum, Plasma, Cell Lysates, Tissue
Maximum samples to be used with this kit 40 samples in duplicate
Assay duration 30 minutes
Kit's sensitivity 0,04 ng/ml
Assay detection limit 0,2 - 13 ng/ml
Storage recommendations 4°C
Shipping recommendations Blue Ice
Bibliography 1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394(6693): 595-599. 2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19):1558-70. 5. Sarto C., Binnz P.A., and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 6. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6.
Release date 2-Feb-2011
PubMed number 27112486
Tested applications ELISA
Tested reactivity Human
Resources available upon request Kit Booklet, MSDS
Product image legend Typical Standard Curve for the HSP27 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-109. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.2 - 13 ng/mL. Graph of the Typical Standard Curve for the HSP27 ELISA Kit StressXpress - SKT-109
Warnings Non-hazardous
Country of production Canada
Total weight (kg) 2
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetHSP27
Short name HSP27 ELISA Kit
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Alternative name HSP27 Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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