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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
HPV58 E6
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Specificity
This antibody will also detect E6 in HPV33 and Alphapapillomavirus 9.HPV58 E6
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic peptide derived from HPV58 E6 protein
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Gene ID
N/A
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Virus
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Cross Reactive Species details
HPV58
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Background information
Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro. Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
E6 protein [Human papillomavirus type 58]; E6 protein HPV58; Human Papilloma Virus; Human papillomavirus type 58; Human papillomavirus type 58; Protein 58; HPV58-E6 protein; HPV58 E6 protein; VE6_HPV58.
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Also known as
HPV58 E6 Polyclonal Antibody
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Other name
Anti-HPV58 E6 Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
