Mouse anti CD27

• Catalog number
  0271
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  The CD27 antigen recognizes disulfide-linked dimer nerve growth factor (NGF) super family of cells expressing the 55kD surface antigen. CD27 acts as a co-stimulatory on T lymphocytes with its ligand of CD70.
• Antibody come from
  n/a
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
• Clone
  DEN-3
• Antigen antibody binding interaction
  Mouse anti CD27 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Research interest
  CD Marker
• Application
  Flow Cytometry
• Antibody s suited for
  PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Storage
  -20ºC
• Relevant references
  1. Morimoto C., Cluster report : CD27. In Schlossman SF., Boumsell L., Gilks W., et al., eds. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press: 1995:356-357. _x000B__x000B_2. CD27 induction on thymocytes. J Immunol 1990 Sep ;145(5):1356-63 Martorell J; Rojo I; Vilella R; Martinez-Caceres E; Vives J _x000B__x000B_3. CD27 expression by a distinct subpopulation of human B lymphocytes. Eur J Immunol 1990 De;20(12):2679-84 Maurer D; Holter W; Majdic O; Fischer GF; Knapp W _x000B__x000B_4. Regulation of expression of CD27, a T cell-specific member of a novel family of membrane receptors. J Immunol 1991 Apr 1;146(8):2488-94 de Jong R; Loenen WA; Brouwer M; van Emmerik L; de Vries EF; Borst J; van Lier RA _x000B__x000B_5. The T-cell activation antigen CD27 is a member of the growth factor/tumor necrosis factor receptor gene family. Camerini D., Walz G., Loenen W., Borst J., Seed B. J. Immunol. 1991:147(9):3165-3169
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD27  
• Gene symbol
  CD27-AS1, CD27
• Short name
  Mouse anti CD27
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to CD27 molecule
• Alternative technique
  murine, antibodies
• Alternative to gene target
  CD27 molecule, S152 and S152. LPFS2 and T14 and TNFRSF7 and Tp55, CD27 and IDBG-14014 and ENSG00000139193 and 939, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, Extracellular, Cd27 and IDBG-189750 and ENSMUSG00000030336 and 21940, CD27 and IDBG-640494 and ENSBTAG00000014725 and 512514

Gene info

• Identity
  HGNC:43896
• Gene
  CD27-AS1
• Long gene name
  CD27 antisense RNA 1
• Synonyms gene name
  • CD27 antisense RNA 1 (non-protein coding)
• GenBank acession
  • AK056500
• Locus
  12p13.31
• Discovery year
  2012-04-27
• Entrez gene record
  678655
• RefSeq identity
  • NR_015382
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000168315

Gene info

• Identity
  HGNC:11922
• Gene
  CD27
• Long gene name
  CD27 molecule
• Synonyms gene
  • TNFRSF7
• Synonyms gene name
  • tumor necrosis factor receptor superfamily, member 7
• Synonyms
  • S152
  • Tp55
• GenBank acession
  • M63928
• Locus
  12p13.31
• Discovery year
  1992-06-10
• Entrez gene record
  939
• Pubmed identfication
  • 2442250
  • 8530100
• Classification
  • Tumor necrosis factor receptor superfamily
  • CD molecules
• VEGA ID
  OTTHUMG00000168316
• Locus Specific Databases
  • LRG_357

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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