S100 ELISA kit
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Catalog number55R-70885
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PricePlease ask
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Size96 wells
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CategoryResearch kit
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Area of researchCancer
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StorageStore at 2-8 deg C
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Shipping conditionsBlue Ice
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Tested forELISA
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Usage RecommendationsOptimal conditions to be determined by end user
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Assay InformationThis S100 EIA is a solidphase, twostep, noncompetitive immunoassay based on two mouse monoclonal antibodies specific for two different epitopes expressed in S100B. The assay determines both S100A1B and S100BB without crossreactivity with other forms of S100. Calibrators and samples are incubated together with biotinylated AntiS100B monoclonal antibody (MAb) S23 in Streptavidin coated microstrips. S100B present in calibrators or samples is adsorbed to the Streptavidin coated microwells by the biotinylated AntiS100B MAb during the incubation. The strips are then washed and incubated with horseradish peroxidase (HRP) labelled AntiS100B MAb S53. After washing, buffered Substrate/Chromogen reagent (hydrogen peroxide and 3, 3’, 5, 5’ tetramethylbenzidine) is added to each well and the enzyme reaction is allowed to proceed. During the enzyme reaction a blue colour will develop if antigen is present. The intensity of the colour is proportional to the amount of S100B present in the samples. The colour intensity is determined in a microplate spectrophotometer at 620 nm (or optionally at 405 nm after addition of Stop Solution). Calibration curves are constructed for each assay by plotting absorbance value versus the concentration for each calibrator. The S100B concentrations of samples are then read from the calibration curve.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbolS100A1, S100Z, S100A8, S100A9
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Short nameS100 ELISA kit
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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Alternative nameELISA kit for the detection of S100 in the research laboratory
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein A1
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Synonyms gene
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Synonyms gene name
- S100 calcium-binding protein A1
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-07-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein Z
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Synonyms gene name
- S100 calcium binding protein, zeta
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-09-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein A8
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Synonyms gene
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Synonyms gene name
- calgranulin A
- cystic fibrosis antigen
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-05-19
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Entrez gene record
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameS100 calcium binding protein A9
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Synonyms gene
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Synonyms gene name
- calgranulin B
- S100 calcium-binding protein A9 (calgranulin B)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-05-19
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Entrez gene record
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RefSeq identity
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Classification
- S100 calcium binding proteins
- EF-hand domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data