Mouse Monoclonal IgG1 Anti-N-CAM, Neuronal Cell Adhesion Molecule [RNL-1]

• Catalog number
  NC100
• Price
  Please ask
• Size
  1 ml

• Availability
  Please contact us to check the availability
• Immunogen
  NCI-H82 cell preparation
• Specificity
  Extracellular domain of the N-CAM molecule (200, 100 and 25-27 kDa proteins of NCI-H82 SCLC cell line in western blot)
• Species reactivity
  Human
• Product presentation
  Cell culture supernatant containing 0.09% sodium azide as preservative
• Product category
  Primary Antibody
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG1
• Antibody s clone
  RNL-1
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  1:10-1:25
• Protein concentration
  N/A
• Positive control
  Pancreas islets or adrenal medulla
• Tested applications
  IHC(C), suitability for paraffin sections not established. Glutaraldehyde fixation for EM has been used successfully (Broers et al. 1991).
• Recommended secondary reagent
  We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
• Storage method
  Store at 2-8°C
• UniProt number
  Go to UniProt/SwissProt
• Litterature
  1. Broers V.J.L., Mijnheere E.P., Rot M.K., Schaart G. Sijlmans A., Boerman O.C., and Raaemaekers F.C.S. (1991) Novel antigens charakteristic of neuroendocrine malignancies. Cancer 67,3; 619-633. 2. Boerman O.C., Mijnheere E.P., Broers J.L.V., Vooijs G.P., and Ramaekers F.C.S. (1991) Biodistribution of a monoclonal antibody (RNL-1) against the neural cell adhesion molecule (NCAM) in athymic mice bearing human small-cell lung-cancer xenografts. Int. J. Cancer 48; 457-462. 3. Stahel R.A., Gilks W.R., Schenker Th., and Gerardy-Schahn R. (1995) NK3.1 Third International Workshop on Lung Tumour and Differentiation Antigens: summary and report on NCAM studies. In: Leucocyte Typing V. Schlossman S.F. et al. eds. Oxford University Press, Oxford, N.Y. Tokyo.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Other products
• Description
  cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Research. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG1   N-CAM   Neuronal   Adhesion   Molecule   [RNL-1]  
• Gene symbol
  KRIT1, CAMK2N2, NCAM1, GH1, H2AC17, POLM, CFAP251, NRAS, CFAP91, CFAP61
• Short name
  Research
• Technique
  Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1
• Species
  Mouse, Mouses
• Alternative name
  N-CAM, Neuronal Cell Adhesion Molecule
• Alternative technique
  murine, antibodies
• Tissue
  cell

Gene info

• Identity
  HGNC:1573
• Gene
  KRIT1
• Long gene name
  KRIT1 ankyrin repeat containing
• Synonyms gene
  • CCM1
• Synonyms gene name
  • cerebral cavernous malformations 1
  • KRIT1, ankyrin repeat containing
• Synonyms
  • CAM
• GenBank acession
  • AJ294850
• Locus
  7q21.2
• Discovery year
  1995-06-02
• Entrez gene record
  889
• Pubmed identfication
  • 7604043
  • 11342228
• Classification
  • FERM domain containing
  • Ankyrin repeat domain containing
  • CCM adhesion complex
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000131187
• Locus Specific Databases
  • LRG_650

Gene info

• Identity
  HGNC:24197
• Gene
  CAMK2N2
• Long gene name
  calcium/calmodulin dependent protein kinase II inhibitor 2
• Synonyms
  • CaM-KIIN
• GenBank acession
  • AY037149
• Locus
  3q27.1
• Discovery year
  2005-06-10
• Entrez gene record
  94032
• Pubmed identfication
  • 11444830
  • 9724800
• RefSeq identity
  • NM_033259
• VEGA ID
  OTTHUMG00000156821

Gene info

• Identity
  HGNC:7656
• Gene
  NCAM1
• Long gene name
  neural cell adhesion molecule 1
• Synonyms
  • NCAM
  • CD56
• Locus
  11q23.2
• Discovery year
  2001-06-22
• Entrez gene record
  4684
• RefSeq identity
  • NM_000615
• Classification
  • Fibronectin type III domain containing
  • CD molecules
  • Ig-like cell adhesion molecule family
  • I-set domain containing
• VEGA ID
  OTTHUMG00000167196

Gene info

• Identity
  HGNC:4261
• Gene
  GH1
• Long gene name
  growth hormone 1
• Synonyms
  • GH-N
  • GHN
  • GH
  • hGH-N
• Synonyms name
  • pituitary growth hormone
  • somatotropin
• GenBank acession
  • M13438
• Locus
  17q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  2688
• Pubmed identfication
  • 6306568
• RefSeq identity
  • NM_000515
• Classification
  • Growth hormone family
• VEGA ID
  OTTHUMG00000172293

Gene info

• Identity
  HGNC:4735
• Gene
  H2AC17
• Long gene name
  H2A clustered histone 17
• Synonyms gene
  • H2AFN
  • HIST1H2AM
• Synonyms gene name
  • H2A histone family, member N
  • histone 1, H2am
  • histone cluster 1, H2am
  • histone cluster 1 H2A family member m
• Synonyms
  • H2A/n
  • H2A.1
• GenBank acession
  • X57138
• Locus
  6p22.1
• Discovery year
  1998-08-27
• Entrez gene record
  8336
• Pubmed identfication
  • 1768865
  • 9439656
  • 12408966
• RefSeq identity
  • NM_003514
• Classification
  • H2A histones
• VEGA ID
  OTTHUMG00000014494

Gene info

• Identity
  HGNC:9185
• Gene
  POLM
• Long gene name
  DNA polymerase mu
• Synonyms gene name
  • polymerase (DNA directed), mu
  • polymerase (DNA) mu
• Synonyms
  • Tdt-N
• Synonyms name
  • Pol iota
• GenBank acession
  • AF176097
• Locus
  7p13
• Discovery year
  2000-02-16
• Entrez gene record
  27434
• Pubmed identfication
  • 10982892
• RefSeq identity
  • NM_013284
• Classification
  • DNA polymerases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000155353

Gene info

• Identity
  HGNC:28506
• Gene
  CFAP251
• Long gene name
  cilia and flagella associated protein 251
• Synonyms gene
  • WDR66
• Synonyms gene name
  • WD repeat domain 66
• Synonyms
  • MGC33630
  • CaM-IP4
• GenBank acession
  • AL833930
• Locus
  12q24.31
• Discovery year
  2005-05-26
• Entrez gene record
  144406
• Pubmed identfication
  • 17967944
• RefSeq identity
  • NM_144668
• Classification
  • WD repeat domain containing
• VEGA ID
  OTTHUMG00000168948

Gene info

• Identity
  HGNC:7989
• Gene
  NRAS
• Long gene name
  NRAS proto-oncogene, GTPase
• Synonyms gene name
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • neuroblastoma RAS viral oncogene homolog
• Synonyms
  • N-ras
• GenBank acession
  • BC005219
• Locus
  1p13.2
• Discovery year
  2001-06-22
• Entrez gene record
  4893
• Pubmed identfication
  • 6705568
  • 11524732
• RefSeq identity
  • NM_002524
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000012059
• Locus Specific Databases
  • NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
  • LRG_92

Gene info

• Identity
  HGNC:24010
• Gene
  CFAP91
• Long gene name
  cilia and flagella associated protein 91
• Synonyms gene
  • C3orf15
  • MAATS1
• Synonyms gene name
  • chromosome 3 open reading frame 15
  • MYCBP/AMY-1-associated, testis expressed 1
  • MYCBP-associated, testis expressed 1
  • MYCBP associated and testis expressed 1
• Synonyms
  • AAT1
  • AAT1alpha
  • SPATA26
  • CaM-IP2
  • AAT-1
• Synonyms name
  • AMY-1-associating protein expressed in testis 1
  • MYCBP-binding protein
  • spermatogenesis associated 26
• GenBank acession
  • AB063296
• Locus
  3q13.33
• Discovery year
  2004-07-26
• Entrez gene record
  89876
• Pubmed identfication
  • 12223483
  • 14551891
  • 17967944
• RefSeq identity
  • NM_033364
• VEGA ID
  OTTHUMG00000159422

Gene info

• Identity
  HGNC:15872
• Gene
  CFAP61
• Long gene name
  cilia and flagella associated protein 61
• Synonyms gene
  • C20orf26
• Synonyms gene name
  • chromosome 20 open reading frame 26
• Synonyms
  • DKFZP434K156
  • dJ1002M8.3
  • CaM-IP3
• Synonyms name
  • hypothetical protein LOC26074
• GenBank acession
  • AL117439
• Locus
  20p11.23
• Discovery year
  2001-06-21
• Entrez gene record
  26074
• Pubmed identfication
  • 11780052
  • 17967944
• RefSeq identity
  • NM_015585
• Classification
  • Cilia and flagella associated
• VEGA ID
  OTTHUMG00000032006

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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