M-MuLV Reverse Transcriptase, 200U/ul
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Catalog number
A1111
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Price
Please ask
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Size
5000U
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Chemical grade
Molecular Biology
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Storage requirements
-20 °C
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Category
DNA & PCR
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Hazardous number
Please see datasheet or contact us for more information and details about hazardous materials.
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Chemical class
Please see datasheet
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Chemical PG
No Data Available
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Supplementary technical information
Contact us for more technical information
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Notes
For research use only. Not for diagnostic procedures.
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Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goup
reverse transcription
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Gene target
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Short name
M-MuLV Reverse Transcriptase, 200U/ul
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Technique
reverse
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Alternative name
M-MuLV Reverse Transcriptase, 200U/ul
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Alternative technique
transctription
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MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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