Mouse Anti-Human PARP Monoclonal IgG1, Clone M3

  • Catalog number
    SMC-259D-A390
  • Price
    Please ask
  • Size
    100 µl
  • Clone
    M3
  • Immunogen
    A synthesized peptide derived from human PARP
  • Antibody s full description
    Mouse Anti-Human PARP Monoclonal IgG1 Antibody, Clone: M3: ATTO 390
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody, ADP ribosyltransferase diphtheria toxin like 1 antibody, ADP ribosyltransferase NAD(+) antibody, ADPRT 1 antibody, ADPRT antibody, ADPRT1 antibody, ARTD1 antibody, msPARP antibody, NAD(+) ADP ribosyltransferase 1 antibody, NAD(+) ADP-ribosyltransferase 1 antibody, pADPRT 1 antibody, pADPRT1 antibody, PARP 1 antibody, PARP antibody, PARP-1 antibody, PARP1 antibody, PARP1_HUMAN antibody, Poly (ADP ribose) polymerase 1 antibody, poly (ADP ribose) polymerase family, member 1 antibody, Poly [ADP-ribose] polymerase 1 antibody, Poly(ADP ribose) polymerase antibody, poly(ADP ribose) synthetase antibody, poly(ADP ribosyl)transferase antibody, Poly[ADP ribose] synthetase 1 antibody, Poly[ADP-ribose] synthase 1 antibody, PPOL antibody
  • Raised in
    Mouse
  • Antibody s target
    PARP
  • Brandname
    None
  • Antibodies applications
    WB
  • Antibody s reactivity
    Human
  • Antibody s dilutions
    WB (1:2000); optimal dilutions for assays should be determined by the user.
  • Purity
    Peptide Affinity Purified
  • Antibody buffer for storage
    PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
  • Antibody s specificity
    Antibody can detects endogenous PARP protein.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:2000 dilution of SMC-259 was sufficient for detection of PARP in 10 µg of Human HeLa, 293T, Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes involving mainly DNA repair and programmed cell death. It is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
  • Bibliography
    1. Piskunova TS. (2008) Curr Gerontol Geriatr Res: 754190. 2. Espinoza LA. et al. (2007) Free Radic. Biol. Med. 42 (9): 1430–40. 3. Zerfaoui M. et al. (2008) Cell. Signal. 20 (1): 186–94.
  • Released date
    06-Sep-2016
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001609.2
  • Gene number
    142
  • Protein number
    P09874
  • Antibody s datasheet
    Contact our support service
  • Representative figure legend
    Western Blot analysis of Human HeLa, 293T, Jurkat cell lysates showing detection of ~113 kDa PARP protein using Mouse Anti-PARP Monoclonal Antibody, Clone M3 (SMC-259). Lane 1: Human HeLa. Lane 2: Human 293T. Lane 3: Human Jurkat.. Primary Antibody: Mouse Anti-PARP Monoclonal Antibody (SMC-259) at 1:2000. Predicted/Observed Size: ~113 kDa. Mouse Anti-PARP Antibody [M3] used in Western Blot (WB) on Human HeLa, 293T, Jurkat cell lysates (SMC-259)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
    PARP   Monoclonal   IgG1  
  • Gene symbol
    TNKS2, TIPARP, PARP1, PARP12, TNKS
  • Short name
    Mouse Anti- PARP Monoclonal IgG1, Clone M3
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 390
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman PARP monoclonal IgG1, clonality M3
  • Alternative technique
    murine, antibodies
  • Clone name
    M3
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    poly(ADP-ribose) polymerase 1
  • Synonyms gene
  • Synonyms gene name
    • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
    • poly (ADP-ribose) polymerase family, member 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
    142
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Zinc fingers PARP-type
    • Zinc fingers
    • Poly(ADP-ribose) polymerases
  • VEGA ID
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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