Composition 75% v/v aqueous Ethanol 99.500 ml Sodium chloride 0.500 gm Hydrochloric acid 0.500 ml
Description Mycobacteria decolourizer is a decolourizing solution reported to be used in the fluorescent staining of mycobacteria.
Appearance Colourless solution.
Clarity Clear without any particles.
Storage Store below 30°C in tightly closed container and away from bright light.
Results Acid fast bacilli are observed as luminous yellow rods in dark field.
Classification Mycobacterial Stains
Shelf Life Use before expiry date on label.
Data Sheet NA
Microscopic Examination Fluorescent staining is carried out using acid alcohol as an intermediate step for decolourization and staining is observed under fluorescence microscope. Slides were screened under high power (400 X) and verified under oil immersion lens.
Application For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
Short name Mycobacteria decolourizer
Alternative name Mycobacteria decolourizer
Alternative technique elisa