Rabbit Anti-Human TFIIF (pSer385 + pThr389) Polyclonal: APC

Catalog number

SPC-991D-APC
Price

Please ask
Size

100 µl

Immunogen

A phospho-specific peptide corresponding to residues surrounding Ser385 and Thr389 of human TFIIF (AA382-392)
Antibody s target

Human TFIIF (pSer385 + pThr389)
Antibody s full description

Rabbit Anti-Human TFIIF (pSer385 + pThr389) Polyclonal
Antibody s category

Polyclonal Antibodies
Antibody s other name

GTF2F1 Antibody, Transcription initiation factor IIF subunit alpha Antibody, 2810405L04Rik Antibody, BTF4 Antibody, C76800 Antibody, General transcription factor IIF 74 kDa subunit Antibody, MGC94148 Antibody, OTTHUMP00000237859 Antibody, RAP74 Antibody, T2FA_HUMAN Antibody, TF2F1 Antibody, GTF2F1 Antibody, TFIIF-alpha Antibody, Transcription initiation factor RAP74 Antibody
Verified applications

WB, AM
Raised in

Rabbit
Antibody s reactivity

Human
Antibody s recommended dilutions for use

WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody s purified from

Peptide Affinity Purified
Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody s concentration

See included datasheet or contact our support service
Antibody s specificity

Detects 58.24 kDa.
Storage recommendations

-20°C
Shipping recommendations

Blue Ice or 4°C
Certificate of analysis

A 1:250 dilution of SPC-991 was sufficient for detection of TFIIF (pSer385 + pThr389) in 10 µg of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell

Nucleus
Tissue specificity

See included datasheet or contact our support service.
Scientific context

TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
Bibliography

Refer to PubMed
Released date

29/Aug/2016
NCBI number

NP_002087
Gene number

2962
Protein number

P35269
PubMed number

Refer to PubMed
Tested applications

To be tested
Tested reactivity

To be tested
Antibody s datasheet

Contact our support service to receive datasheet or other technical documentation.
Representative figure link

No Data Available
Warning information

Non-hazardous
Country of production

Canada
Total weight kg

1.4
Net weight g

0.1
Stock availabilit

In Stock
Other related products
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Test

StressMark antibodies supplies antibodies that are for research of human proteins.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name

Oryctolagus cuniculus

Gene target

TFIIF pSer385 pThr389 APC
Gene symbol

GTF2F2P1, GTF2F1, APC, GTF2F2
Short name

Rabbit Anti- TFIIF (pSer385 + pThr389) Polyclonal: APC
Technique

anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Host

Rabbit, Rabbits
Isotype

Immunoglobulin
Label

APC
Species

Human, Humans
Alternative name

production species: rabbit Antibody toHuman TFIIF (pSer385 + pThr389) polyclonal: adenomatous polyposis coli
Alternative technique

polyclonals, rabbit-anti, antibodies
Alternative to gene target

adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233

Gene info

Identity

HGNC:4654
Gene

GTF2F2P1
Long gene name

general transcription factor IIF subunit 2 pseudogene 1
Synonyms gene
- GTF2F2L
Synonyms gene name
- general transcription factor IIF, polypeptide 2-like
- general transcription factor IIF, polypeptide 2 pseudogene 1
Synonyms
- TFIIF
Locus

4q31.23
Discovery year

1994-07-26
Entrez gene record

2964
Pubmed identfication
- 7835093
RefSeq identity
- NG_003013
VEGA ID

OTTHUMG00000161355

Gene info

Identity

HGNC:4652
Gene

GTF2F1
Long gene name

general transcription factor IIF subunit 1
Synonyms gene name
- general transcription factor IIF, polypeptide 1 (74kD subunit)
- general transcription factor IIF, polypeptide 1, 74kDa
Synonyms
- TFIIF
- BTF4
- RAP74
- TF2F1
Locus

19p13.3
Discovery year

1992-11-04
Entrez gene record

2962
Pubmed identfication
- 1734284
RefSeq identity
- NM_002096
Classification
- General transcription factor IIF complex subunits
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000168087

Gene info

Identity

HGNC:583
Gene

APC
Long gene name

APC regulator of WNT signaling pathway
Synonyms gene name
- adenomatosis polyposis coli
- adenomatous polyposis coli
- APC, WNT signaling pathway regulator
Synonyms
- DP2
- DP3
- DP2.5
- PPP1R46
Synonyms name
- protein phosphatase 1, regulatory subunit 46
GenBank acession
- M74088
Locus

5q22.2
Discovery year

1986-01-01
Entrez gene record

324
Pubmed identfication
- 1651563
RefSeq identity
- NM_000038
Classification
- Protein phosphatase 1 regulatory subunits
- Armadillo repeat containing
VEGA ID

OTTHUMG00000128806
Locus Specific Databases

Gene info

Identity

HGNC:4653
Gene

GTF2F2
Long gene name

general transcription factor IIF subunit 2
Synonyms gene name
- general transcription factor IIF, polypeptide 1 (74kD subunit)
- general transcription factor IIF, polypeptide 2, 30kDa
Synonyms
- TFIIF
- BTF4
- RAP30
GenBank acession
- X16901
Locus

13q14.12-q14.13
Discovery year

1992-11-04
Entrez gene record

2963
Pubmed identfication
- 8162052
RefSeq identity
- NM_004128
Classification
- General transcription factor IIF complex subunits
VEGA ID

OTTHUMG00000016849

MeSH Data

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Rabbit Anti-Human TFIIF (pSer385 + pThr389) Polyclonal: APC

Gene info

Gene info

Gene info

Gene info

MeSH Data

Product images

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