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Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr251 of human PDLIM5 (AA248-254)
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Antibody s target
Human PDLIM5 (pTyr251)
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Antibody s full description
Rabbit Anti-Human PDLIM5 (pTyr251) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
ENH Antibody, ENH1 Antibody, Enigma homolog Antibody, Enigma like LIM domain protein Antibody, Enigma like PDZ and LIM domains protein Antibody, Enigma-like PDZ and LIM domains protein Antibody, L 9 Antibody, L9 Antibody, LIM Antibody, PDLI5_HUMAN Antibody, PDLIM5 Antibody, PDZ and LIM domain 5 Antibody, PDZ and LIM domain protein 5 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 64.002 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1051 was sufficient for detection of PDLIM5 (pTyr251) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cell Junction, Synapse, Postsynaptic cell membrane, Postsynapticdensity, Synaptosome, Cytoplasm
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Tissue specificity
Highly expressed in heart and skeletal muscle. Expression is commonly increased in the brain of patients with bipolar disorder, schizophrenia, and major depression.
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Scientific context
See included datasheet or contact our support service.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_001011513
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Gene number
10611
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Protein number
Q96HC4
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
PDLIM5, APC
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Short name
Rabbit Anti- PDLIM5 (pTyr251) Polyclonal: APC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
APC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman PDZ and LIM domain 5 (pTyr251) polyclonal: adenomatous polyposis coli
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
PDZ and LIM domain 5, ENH and ENH1 and LIM, PDLIM5 and IDBG-30475 and ENSG00000163110 and 10611, protein N-terminus binding, Cell surfaces, Pdlim5 and IDBG-197000 and ENSMUSG00000028273 and 56376, PDLIM5 and IDBG-641022 and ENSBTAG00000012818 and 503621
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