Rabbit Anti Human WDFY3 Antibody-Biotin

• Catalog number
  CSB-PA809030LD01HU_50ug
• Price
  Please ask
• Size
  50ug

• Long name
  Rabbit Antibody against Human WDFY3, Biotin
• Synonym name
  WDFY3 Human Antibody
• Species reactivity
  Human
• Tested applications recommended dilutions
  ELISA
• Conjugation
  Biotin
• Antibody isotype
  IgG
• UniProt ID
  Q8IZQ1
• Format
  Liquid, diluted in buffer
• Buffer composition
  Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity
  >95%
• Purification method
  Protein G purified
• Antibody category
  Polyclonal Antibodies
• Immunogen type
  Recombinant Human WD repeat and FYVE domain-containing protein 3 protein (1-100AA)
• Immunogen species
  Human
• Alias
  WD repeat and FYVE domain-containing protein 3, Autophagy-linked FYVE protein, Alfy, WDFY3, KIAA0993
• Background
  Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.
• Clonality
  Polyclonal antibody
• Shipping and storage
  WDFY3 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
• Short name of the antigen
  WD repeat and FYVE domain-containing protein 3
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional conjugation
  Biotinylated
• About
  Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

• Gene target
  WDFY3  
• Gene symbol
  WDFY3-AS1, WDFY3, WDFY3-AS2
• Short name
  Anti-WDFY3
• Technique
  anti human, Antibody, Rabbit, anti, antibody to, anti human antibodies, antibodies against human proteins, antibodies for
• Host
  Rabbit, Rabbits
• Label
  Biotin
• Species
  Human, Humans
• Alternative name
  WDFY3 Antibody,Biotin conjugated
• Alternative technique
  antibodies, rabbit-anti

Gene info

• Identity
  HGNC:40935
• Gene
  WDFY3-AS1
• Long gene name
  WDFY3 antisense RNA 1
• Synonyms gene name
  • WDFY3 antisense RNA 1 (non-protein coding)
• Locus
  4q21.23
• Discovery year
  2011-08-11
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000160941

Gene info

• Identity
  HGNC:20751
• Gene
  WDFY3
• Long gene name
  WD repeat and FYVE domain containing 3
• Synonyms
  • KIAA0993
  • ALFY
  • ZFYVE25
• GenBank acession
  • AB023210
• Locus
  4q21.23
• Discovery year
  2003-03-28
• Entrez gene record
  23001
• Pubmed identfication
  • 10231032
• RefSeq identity
  • NM_014991
• Classification
  • BEACH domain containing
  • Armadillo like helical domain containing
  • Zinc fingers FYVE-type
  • WD repeat domain containing
• VEGA ID
  OTTHUMG00000130424

Gene info

• Identity
  HGNC:21603
• Gene
  WDFY3-AS2
• Long gene name
  WDFY3 antisense RNA 2
• Synonyms gene
  • C4orf12
  • NCRNA00247
• Synonyms gene name
  • chromosome 4 open reading frame 12
  • non-protein coding RNA 247
  • WDFY3 antisense RNA 2 (non-protein coding)
• Synonyms
  • FBI4
• GenBank acession
  • AY250185
• Locus
  4q21.23
• Discovery year
  2005-06-30
• Entrez gene record
  404201
• Pubmed identfication
  • 15580561
  • 31347170
• RefSeq identity
  • NR_015359
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000141281

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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