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We send this reagant on
Room temperature
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The temperatureerature for storage is
Upon receipt, aliquot and store the Radius™ Gel Removal Solution and DAPI Fluorescence Stain at -20ºC (avoid multiple freeze/thaw cycles), and transfer the Fixation Solution to 4ºC. All other test kit components should be stored at room temperatureerature until the test kit’s expiration date.
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SDS or safty data sheet
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Type of reagent
Cell Based Assays
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Related techniques
Cell Based Assays, Cell Migration, Invasion and Wound Healing, Gap Closure Assays, Cell Migration Assays, 2D Gap Closure
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Use of the reagent
The Radius™ Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius™ assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius™ gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius™ gel, creating a cell-free zone. Following cell seeding the Radius™ gel is removed, allowing migratory cells to move across the area and close the gap.
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Cellular use
Proprietary biocompatible hydrogel creates a circular area across which cells may migrate following gel removal
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Protocol information
Versatile plate format allows use with cells of any size; no need to worry about selecting cell culture inserts with the proper pore size
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Reagent properties
Allows qualitative, quantitative, endpoint or real-time analysis
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Additional lab advice
Adaptable to liquid handling equipment and HCS instrumentation
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Living modified organisme in this reagent or LMO
Not applicable
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,7
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Test
Cellular biology labs supplies other types of Assays as 1.
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Description
For cells, cell lines and tissues in culture till half confluency.