CD3 monoclonal antibody

• Catalog number
  PB-514-T100
• Price
  Please ask
• Size
  100 assays

• Long name
  Mouse Monoclonal Antibody against Human CD3, Clone: UCHT1
• Antigen
  CD3
• Species reactivity
  Human (Homo sapiens), Non-Human (Homo sapiens) Primates
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  UCHT1
• Applications
  FC, IP, IHC(F), MC, FUNC
• Immunogen
  human thymocytes followed by Sezary T cells
• Format conjugation
  Pacific Blue
• Specificity
  The antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex. _x000D_ HLDA I; WS Code T 3_x000D_ HLDA III; WS Code T 126_x000D_ HLDA III; WS Code T 471_x000D_ HLDA VI; WS Code T 6T-CD3.1
• Research area
  CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
• Storage buffer
  The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
• Shipping and storage
  Mouse Monoclonal Antibody against Human CD3, Clone: UCHT1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation._x000D_ The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by affinity chromatography
• Datasheet
• References
  , *Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med. 2006 May 4;354(18):1913-21., *Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1_x000D_ single-chain antibody fragment. Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16268-73., *Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003 Jun 15;170(12):5947-55., *le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase _x000D_ II after CD2 triggering of human T lymphocytes. J Immunol. 1998 Aug 1;161(3):1113-22. , *Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature_x000D_ natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74., *Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol. 2005 Mar;32(3):177-84., *Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinct_x000D_ patterns of non-major histocompatibility complex-restricted cytolysis. J Exp Med. 1990 May 1;171(5):1567-79._x000D_ _x000D_ _x000D_ , *Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor_x000D_ explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med. 2007 Oct 29;204(11):2537-44._x000D_ _x000D_ , *Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982 Jul 22;298(5872):375-7._x000D_ , *Barclay, Brown et al.: The Leukocyte Antigen FactsBook, 2nd edition, Harcourt Brace & Company, London, (1997)., *Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD3   monoclonal  
• Short name
  Anti-CD3 Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to CD3
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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