Rabbit Anti-Human Calreticulin Polyclonal: Streptavidin

  • Catalog number
    SPC-122B-STR
  • Price
    Please ask
  • Size
    200 µl
  • Immunogen
    Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH
  • Antibody s target
    Human Calreticulin
  • Antibody s full description
    Rabbit Anti-Human Calreticulin Polyclonal
  • Primary research fields
    Cell Signaling, Trafficking
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    CALR Antibody, Calregulin Antibody, cC1qR Antibody, CRP55 Antibody, ERp60 Antibody, HSCBP Antibody, RO Antibody, SSA Antibody, grp60 Antibody
  • Verified applications
    WB, IHC, ICC/IF, IP, FCM
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig (Cavia porcellus), Monkey, Pig, Hamster, Rabbit , Sheep
  • Antibody s recommended dilutions for use
    WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~63kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-122 was sufficient for detection of Calreticulin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
  • Antibody in cell
    Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Cytoplasm, Cell Surface, Extracellular Matrix, Sarcoplasmic Reticulum, Sarcoplasmic Reticulum Lumen
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT’s three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).
  • Bibliography
    1. Johnson S., et al. (2001) Trends Cell Biol 11: 122-129. 2. Smith M.J., et al. (1989) EMBO J. 8: 3581-3586. 3. Ellgaard L., et al. (2001) Curr Opin Cell Biol. 13: 431-437. 4. Krause K.H., and Michalak M. (1997) Cell. 88: 439-443. 5. Nash P.D., et al. (1994) Mol Cell Biochem. 135: 71-78. 6. Heal R., and McGivan J. (1998) Biochem J. 329: 389-394. 7. Lucero H.A., et al. (1998) J Biol Chem. 273: 9857-9863. 8. Tanaka S., et al. (2000) J Biol Chem. 275: 10388-10393. 9. Yoon G.S., et al. (2000) Cancer Res. 60: 1117-1120. 10. Antoniou A.N., et al. (2002) Immunology 106: 182-189. 11. Wada I., et al. (1997) EMBO J. 16: 5420-5432. 12. Laguerre D.B., et al. (1998) J Vir. 72: 4940-4949.
  • Released date
    1/May/2008
  • NCBI number
    NP_004334.1
  • Gene number
    811
  • Protein number
    P27797
  • PubMed number
    27170757|25525794|25070849|24792671|25036135|19940990
  • Tested applications
    Immunocytochemistry/Immunofluorescence|Immunocytochemistry/Immunofluorescence|Flow Cytometry|Western Blot|Western Blot|Western Blot
  • Tested reactivity
    Human|Human|Mouse|Mouse|Rat|Mouse
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of multiple cell lines lysates showing detection of Calreticulin protein using Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122) at 1:5000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic granule. Endoplasmic reticulum lumen. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody (SPC-122) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-Calreticulin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-122) | Rabbit Anti-Calreticulin Antibody used in Western blot (WB) on multiple cell lines lysates (SPC-122) | Rabbit Anti-Calreticulin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-122) | Rabbit Anti-Calreticulin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-122)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.2
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Short name
    Rabbit Anti- Calreticulin Polyclonal: Streptavidin
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    Streptavidin
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman Calreticulin polyclonal: biotinilated-binding streptavidin
  • Alternative technique
    polyclonals, rabbit-anti, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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