CD28 FITC, Single-Color Reagent
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Catalog number
0282
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Price
Please ask
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Size
100Tests
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Host Source
Mouse
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Clone Name
B-23
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Product Category
Flow Cytometry
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Species Reactivity
Human
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Application
Flow Cytometry
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Immunogen
CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
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Purification Method
Protein A/G Chromatography
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Shipping Conditions
Room Temperature
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Storage conditions
CD28 FITC, Single-Color Reagent should be stored at 4-8ÂșC. DO NOT FREEZE
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
CD28
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Short name
CD28 FITC, Single-Color Reagent
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Technique
FITC, Fluorescein
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Isotype
IgG1
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Label
FITC
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Alternative name
CD28 molecule fluorecein, Single-Color Reagent
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Alternative technique
fluorescine
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Alternative to gene target
CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
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Gene info
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Identity
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Gene
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Long gene name
CD28 molecule
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Synonyms gene name
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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