Early B-Cell Factor 1 (EBF1) Polyclonal Antibody (Human, Rat), Cy3

  • Catalog number
    PAB422Hu01-200ul-Cy3
  • Price
    Please ask
  • Size
    200ul
  • Description
    A Rabbit polyclonal antibody against Human, Rat Early B-Cell Factor 1 (EBF1). This antibody is labeled with Cy3.
  • Specifications
    Host: Rabbit; Species Reactivity: Human, Rat; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: Cy3
  • Additional_information
    Sequence of the immunogen: EBF1 (Asp179~Ser451); Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Storage_and_shipping
    Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
  • Notes
    Research Use Only.
  • Properties
    If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Early B- Factor 1 (EBF1) Polyclonal Antibody ( , ), can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Cloud Clone Corp antibody is well suited for fluorescent microscopy. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional description
    For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Conjugation
    cy3 conjugation kit
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • French translation
    anticorps
  • Gene target
    Early   Factor   EBF1  
  • Gene symbol
    EBF1
  • Short name
    Early B- Factor 1 (EBF1) Polyclonal Antibody ( , ),
  • Technique
    Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rat
  • Label
    Cy3
  • Species
    Rat, Humans, Rats
  • Alternative name
    Early B-cellular Factor 1 (early B-cell factor 1) polyclonal (antibody to-) (H. sapiens, Rat), cyanine 3
  • Alternative technique
    polyclonals, antibodies
  • Alternative to gene target
    early B-cell factor 1, COE1 and EBF and O/E-1 and OLF1, EBF1 and IDBG-55765 and ENSG00000164330 and 1879, C2H2 zinc finger domain binding, nuclei, Ebf1 and IDBG-164722 and ENSMUSG00000057098 and 13591, EBF1 and IDBG-646416 and ENSBTAG00000037508 and 537712
  • Tissue
    cell
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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