Goat anti Rat IgG H + L Fab'2 PE
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Catalog number
43C-CJ0306
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Price
Please ask
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Size
500 µg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Goat anti-rat IgG (H + L) (Fab'2) (PE) was raised in goat using rat IgG whole molecule ..
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Raised in
Goat
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Specificity
Rat IgG (H + L)
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
PE
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Method of Purification
Goat anti Rat IgG (H + L) (Fab'2) (PE) was purified by affinity chromatography.
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Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.05% NaN3. I mMunoglobulin and protease free.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
IF
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Usage Recommendations
IF: 1:100-1:250
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent
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Latin name
Capra aegagrus hircus, Rattus norvegicus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Short name
Goat anti IgG H + L Fab'2 PE
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Technique
anti, Goat, Fab, IgG, antibody to, fabs, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
fab'2, IgG, Fab fragment, IgG
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Label
PE
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Species
Rat, Goats, Rats
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Alternative name
Goat anti Rat IgG (H + L) secondary antibody (Fab'2) (PE)
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Alternative technique
antibodies, caprine, seconairy, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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