Mouse anti Cytokeratin 14

  • Catalog number
    MUB0324S
  • Price
    Please ask
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
  • Antibody come from
    SPK-14 is a Mouse monoclonal IgG1, κ antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a synthetic peptide corresponding to the carboxy-terminal sequence of Human Cytokeratin 14 (KVVSTHEQVLRTKN) coupled to keyhole limpet hemocyanin.
  • Other description
    Each vial contains 1 ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
  • Clone
    SPK-14
  • Antigen antibody binding interaction
    Mouse anti Cytokeratin 14 Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Feline,Human,Swine,Canine
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    SPK-14 reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stRatified and combined epithelia.
  • Research interest
    Cytoskeleton
  • Application
    Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
  • Antibody s suited for
    SPK-14 is useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration.
  • Storage
    Store at 4oC, or in small aliquots at –20oC.
  • Relevant references
    Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline Cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Bonfrer, J. M., Groeneveld, E. M., Korse, C. M., van Dalen, A., Oomen, L. C., and Ivanyi, D. (1994). Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantifiCation of tissue polypeptide antigen recognizes Keratin 18, Tumour Biol 15, 210-22.
  • Protein number
    P02533
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Mouse anti Cytokeratin 14
  • Technique
    Mouse, anti, antibody to, mouses
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to Cytokeratin 14
  • Alternative technique
    murine, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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