Mouse anti Cytokeratin 14 / Keratin K14
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Catalog numberMUB0323S
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PricePlease ask
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Size1 mL
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CategoryPrimary Antibodies
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Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Antibody come fromRCK107 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal preparation of TR146 epithelial cells.
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Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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CloneRCK107
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Antigen antibody binding interactionMouse anti Cytokeratin 14 / Keratin K14 Antibody
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Antibody is raised inMouse
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Antibody s reacts withCanine,Human,Rat,Swine
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityRCK107 reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stRatified and combined epithelia.
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Research interestCytoskeleton
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ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
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Antibody s suited forRCK107 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, flow cytometry and immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:5 – 1:20 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
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StorageStore at 4°C, or in small aliquots at -20°C.
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Relevant referencesWetzels, R. H., Kuijpers, H. J., Lane, E. B., Leigh, I. M., Troyanovsky, S. M., Holland, R., van Haelst, U. J., and Ramaekers, F. C. (1991). Basal cell-specific and hyperprolifeRation-related Keratins in Human breast cancer, Am J Pathol 138, 751-63. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Robben, H., Lane, B., Leigh, I., Plantema, F., and Vooijs, P. (1992). Basal-cell Keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia, Am J Pathol 140, 601-12.Wetzels, R. H., Schaafsma, H. E., Leigh, I. M., Lane, E. B., Troyanovsky, S. M., Wagenaar, S. S., Vooijs, G. P., and Ramaekers, F. C. (1992). Laminin and type VII collagen distribution in different types of Human lung carcinoma: correlation with expression of Keratins 14, 16, 17 and 18, Histopathology 20, 295-303.Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86.Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511.Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with Keratin monoclonal antibodies in canine tissues: urogenital tract, respiRatory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40.Vos, J. H., van den Ingh, T. S., Ramaekers, F. C., Molenbeek, R. F., de Neijs, M., van Mil, F. N., and Ivanyi, D. (1993). The expression of Keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region, Vet Pathol 30, 352-61.van Leenders, G., Dijkman, H., Hulsbergen-van de Kaa, C., Ruiter, D., and Schalken, J. (2000). DemonstRation of intermediate cells during Human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy, Lab Invest 80, 1251-8.
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Protein numberP02533
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse anti Cytokeratin 14 / Keratin K14
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TechniqueMouse, anti, antibody to, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to Cytokeratin 14 / Keratin K14
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Alternative techniquemurine, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data