HM74 (T327) polyclonal antibody

• Catalog number
  BS2605-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  Hydroxycarboxylic acid receptor 3; G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; Niacin receptor 2; Nicotinic acid receptor 2; HCAR3; GPR109B; HCA3; HM74B; NIACR2; Hydroxycarboxylic acid receptor 2; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; Niacin receptor 1; Nicotinic acid receptor; HCAR2; GPR109A; HCA2; HM74A; NIACR1
• Immunogen
  Synthetic peptide, corresponding to amino acids 300-350 of Human HM74.
• Swiss Prot
  P49019/Q8TDS4
• Reactivity
  Human,Mouse,Rat
• Applications
  WB IHC IF
• Application_all
  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  HM74 (T327) polyclonal antibody detects endogenous levels of HM74B protein. This antibody also recognizes HM74A protein.
• Usage
  Western blot (WB) analysis of HM74 (T327) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:The Spleen tissue lysate of Mouse(40ug) Lane5:The Lung tissue lysate of Rat(40ug)
• BiowMW
  ~ 45 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  HM74   T327  
• Gene symbol
  HCAR3
• Short name
  HM74 (T327) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  HM74 (T327) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:16824
• Gene
  HCAR3
• Long gene name
  hydroxycarboxylic acid receptor 3
• Synonyms gene
  • GPR109B
• Synonyms gene name
  • G protein-coupled receptor 109B
• Synonyms
  • HCA3
  • HM74
• GenBank acession
  • D10923
• Locus
  12q24.31
• Discovery year
  2004-04-22
• Entrez gene record
  8843
• Pubmed identfication
  • 7505609
  • 9205127
  • 18983141
  • 21454438
• RefSeq identity
  • NM_006018
• Classification
  • Hydroxycarboxylic acid receptors
• VEGA ID
  OTTHUMG00000166034

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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