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Clone
BH7
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Immunogen
Recombinant human UCHL1 protein
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Antibody s full description
Mouse Anti-Human UCHL1 Monoclonal IgG1 Antibody, Clone: BH7: ATTO 390
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
Ubiquitin Hydrolase Antibody, Ubiquitin carboxy-terminal hydrolase L1 Antibody, Ubiquitin carboxyl-terminal hydrolase isozyme L1 Antibody, PGP9.5 Antibody, PARK5 Antibody
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Raised in
Mouse
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Antibody s target
UCHL1
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Primary research fields
Cell Signaling, Post-Translational Modifications, Ubiquitination, Neuroscience, Neurodegeneration, Parkinson's Disease, Alzheimer's Disease
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Brandname
None
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Antibodies applications
WB, IHC, ICC/IF
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Antibody s reactivity
Human, Mouse, Rat, Bovine, Pig
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Antibody s dilutions
WB (1:20000), IHC (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Ascites
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Antibody buffer for storage
Ascitic fluid containing 10mM sodium azide
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Antibody s specificity
Ubiquitin C-terminal hydrolase enzyme. Neuronal marker protein.
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Storage recommendations
4ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:2000 dilution of SMC-216 was sufficient for detection of UCHL1 in rat spinal cord tissue lysate by chemiluminescence immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Endoplasmic Reticulum , Endoplasmic Reticulum Membrane , Lipid-anchor
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Ubiquitin C-terminal hydrolases (UCHs) are members of the family of deubiquitinating enzymes (DUBs). These enzymes hydrolyze the esters, amides and thioesters on the C-terminal ends of ubiquitin molecules and contribute to ubiquitin stability. UCHL1 is highly expressed in mammalian neuronal cells and therefore, mutations of this enzyme have implications in Parkinson’s disease.
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Bibliography
1. Bilguvar K., Tyagi N., Ozkara C., Tuysuz B., Bakircioglu M., Choi M., et al. (2013). Proc Natl Acad Sci U S A, 110(9): 3489-94.
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Released date
12-Jun-2014
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_004172.2
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Gene number
7345
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Protein number
P09936
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Antibody s datasheet
Contact our support service
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-UCHL1 Monoclonal Antibody, Clone BH7 (SMC-216). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-UCHL1 Monoclonal Antibody (SMC-216) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Lipid-anchor. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UCHL1 Antibody (D) Composite. Mouse Anti-UCHL1 Antibody [BH7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-216)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.05
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
