CD2 FITC - CD20 PE, Bi-Test Reagents (FITC/RPE)

Host Source

Mouse
Clone Name

NA
Product Category

Flow Cytometry
Species Reactivity

Human
Application

Flow Cytometry
Immunogen

CD2=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with t lymphocytes activated by mixed lymphocyte culture.CD20=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.
Purification Method

Protein A/G Chromatography
Shipping Conditions

Room Temperature
Storage conditions

CD2 FITC - CD20 PE, Bi-Test Reagents (FITC/RPE) should be stored at 4-8ºC. DO NOT FREEZE
Datasheet link
Properties

This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

CD2 CD20 Bi-Test Reagents RPE
Gene symbol

RPE, CD2, MS4A1
Short name

CD2 FITC - CD20 PE, Bi-Test Reagents (FITC/RPE)
Technique

FITC, Fluorescein
Isotype

IgG2a (F)/IgG1 (PE)
Label

FITC
Alternative name

CD2 molecule fluorecein - CD20 peroxidase, Bi-Test Reagents (fluorecein/RPE)
Alternative technique

tests, fluorescine
Alternative to gene target

CD2 molecule, LFA-2 and SRBC and T11, CD2 and IDBG-101417 and ENSG00000116824 and 914, protein homodimerization activity, Extracellular, Cd2 and IDBG-179684 and ENSMUSG00000027863 and 12481, CD2 and IDBG-634458 and ENSBTAG00000017256 and 497022

Identity

HGNC:1639
Gene

CD2
Long gene name

CD2 molecule
Synonyms gene
- SRBC
Synonyms gene name
- CD2 antigen (p50), sheep red blood cell receptor
GenBank acession
- BC033583
Locus

1p13.1
Discovery year

1986-01-01
Entrez gene record

914
Pubmed identfication
- 2437578
RefSeq identity
- NM_001767
Classification
- CD molecules
- Ig-like cell adhesion molecule family
- C2-set domain containing
- V-set domain containing
VEGA ID

OTTHUMG00000022750

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data