anti-Pyruvate Dehydrogenase Antibody

• Catalog number
  45-107
• Price
  Please ask
• Size
  0.1 mg

• Gene names
  PDHX, E3BP, OPDX, PDX1, proX, DLDBP, pyruvate dehydrogenase complex, component X, E3-binding protein, lipoyl-containing component X
• Antibodie s label
  unlabelled serum
• Purity
  Pyruvate Dehydrogenase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Ab raised in
  Goat
• Type
  Polyclonal
• Spicific to
  This antibody is expected to recognise all reported isoforms.
• Antigen
  Pyruvate Dehydrogenase antibody was made against a 14 amino acid synthetic peptide near the C-Terminus of Pyruvate Dehydrogenase.
• Immuno application
  ELISA, Western Blot, immuno-histo-chemistry
• Antibody advice
  ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 50 kDa band observed in human pancreas lysate (calculated MW of 54.2 kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approximately 50 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1 ug/mL. Immunohistochemistry: In paraffin embedded human pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3 ug/mL.
• Suspention pH azide
  Pyruvate dehydrogenase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
• Keep at
  Aliquot and store at -20 grades C. Minimize freezing and thawing.
• Works with
  Human
• Omim nr
  4505699
• Ncbi nr
  NP_003468.1
• Vial with antibody
  in solution
• Quantity per ml
  500 ug/mL
• NCBI GENE nr
  8050
• GENE symbol
  PDHX
• Antigene name
  pyruvate dehydrogenase complex, component X
• Source
  Homo sapiens
• Protein nr
  O00330
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Pyruvate   Dehydrogenase  
• Gene symbol
  PDHA2, PDK1P1, PDK4
• Short name
  anti-Pyruvate Dehydrogenase Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toPyruvate Dehydrogenase (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:8807
• Gene
  PDHA2
• Long gene name
  pyruvate dehydrogenase E1 subunit alpha 2
• Synonyms gene
  • PDHAL
• Synonyms gene name
  • pyruvate dehydrogenase (lipoamide) alpha 2
  • pyruvate dehydrogenase alpha 2
• Locus
  4q22.3
• Discovery year
  1990-07-11
• Entrez gene record
  5161
• VEGA ID
  OTTHUMG00000130990

Gene info

• Identity
  HGNC:49808
• Gene
  PDK1P1
• Long gene name
  pyruvate dehydrogenase kinase 1 pseudogene 1
• Synonyms gene name
  • pyruvate dehydrogenase kinase, isozyme 1 pseudogene 1
• Locus
  9p21.1
• Discovery year
  2014-03-06
• Entrez gene record
  100421194
• RefSeq identity
  • NG_023886

Gene info

• Identity
  HGNC:8812
• Gene
  PDK4
• Long gene name
  pyruvate dehydrogenase kinase 4
• Synonyms gene name
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pyruvate dehydrogenase kinase, isozyme 4
• GenBank acession
  • U54617
• Locus
  7q21.3
• Discovery year
  1996-08-14
• Entrez gene record
  5166
• Pubmed identfication
  • 7499431
• RefSeq identity
  • NM_002612
• VEGA ID
  OTTHUMG00000153977

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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