Rat Anti-Drosophila HSP70 Monoclonal IgG2B, Clone 7FB

  • Catalog number
    SMC-230D-BI
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    7FB
  • Immunogen
    Prepared from Drosophila tissue culture cells heat shocked at 36.5◦C for 3 hours, and isolated using SDS PAGE.
  • Antibody s full description
    Rat Anti-Drosophila HSP70 Monoclonal IgG2B Antibody, Clone: 7FB: Biotin
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    HSP70Bb Antibody, Heat Shock Protein 70Bb Antibody, dHSP70 Antibody, HSP70b Antibody, HSP70B Antibody, Dm-HSP70 Antibody
  • Raised in
    Rat
  • Antibody s target
    HSP70
  • Primary research fields
    Cancer, Heat Shock
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Fruit Fly (Drosophila melanogaster)
  • Antibody s dilutions
    WB (1:2000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~70kDa (heat-inducible form).
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-230 was sufficient for detection of Drosophila HSP70 using an indirect assay with rabbit anti-rat IgG and goat anti-rabbit IgG:HRP.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
  • Bibliography
    1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424. 8. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879. 9. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239. 10. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377. 11. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248. 12. Dressel et al. (2000) J. Immunol. 164: 2362-2371. 13. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555. 14. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
  • Released date
    22-Oct-2014
  • PubMed number
    26234525
  • Tested applications
    Western Blot
  • Tested reactivity
    Drosophila
  • NCBI number
    NP_524927.2
  • Gene number
    48582
  • Protein number
    Q9BIS2
  • Antibody s datasheet
    Contact our support service
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
    HSP70   Monoclonal   IgG2B   7FB  
  • Gene symbol
    HSPA1B, HSPA14, HSPA1A, HSPA1L
  • Short name
    Anti- HSP70 Monoclonal IgG2B, Clone 7FB
  • Technique
    anti-, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies
  • Host
    Rat
  • Isotype
    IgG2B, IgG2b
  • Label
    Biotin
  • Species
    Drosophila, Drosophila subspecies d. Megalonaster or the fruit fly is used in genetic research because of the short life cycle and the possibility of knock-out and knock-ins with Cas9 for ends-in gene targets like CRISPR.Cas9 and TALEN vectors . Drosophilas, Rats
  • Alternative name
    Rat Antibody toDrosophila HSP70 monoclonal IgG2B, clonality 7FB
  • Alternative technique
    antibodies
  • Clone name
    7FB
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 1B
  • Synonyms gene name
    • heat shock 70kD protein 1B
    • heat shock 70kDa protein 1B
  • Synonyms
  • Locus
  • Discovery year
    1995-08-11
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • TIM23 complex
    • Heat shock 70kDa proteins
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 14
  • Synonyms gene name
    • heat shock 70kDa protein 14
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2004-04-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Heat shock 70kDa proteins
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 1A
  • Synonyms gene
  • Synonyms gene name
    • heat shock 70kD protein 1A
    • heat shock 70kDa protein 1A
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • Classification
    • TIM23 complex
    • Heat shock 70kDa proteins
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 1 like
  • Synonyms gene name
    • heat shock 70kD protein-like 1
    • heat shock 70kDa protein 1-like
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1989-05-12
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • TIM23 complex
    • Heat shock 70kDa proteins
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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