Recombinant Human Histone Chaperone ASF1A/ASF1A (C-6His, N-T7 tag)
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Catalog numberC290-1000
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PricePlease ask
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Size1 mg
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DescriptionRecombinant Human Histone Chaperone ASF1A is produced by our E.coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMASMTGGQQMGRGSMAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCMLEHHHHHH
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Estimated molecular weight25,4 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionAmbient/Room Temperature
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Package formLyophilized from a 0.2 µm filtered solution of 20mM TrisHCl, 1mM DTT, 150mM NaCl, pH 8.0.
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Storage conditionsLyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberQ9Y294
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolASF1A, GALNT7, GH1, POLM, B4GALT7, NRAS, SDC3, PIGN, WASL, MAP6
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Short nameRecombinant Histone Chaperone ASF1A/ASF1A (C-6His, N-T7 tag)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Labeltag
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SpeciesHuman, Humans
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Alternative nameHuman Histone Chaperone ASF1A(C-6His, N-T7 tag)
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameanti-silencing function 1A histone chaperone
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Synonyms gene name
- ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-05-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolypeptide N-acetylgalactosaminyltransferase 7
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Synonyms gene name
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-09-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Polypeptide N-acetylgalactosaminyltransferases
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegrowth hormone 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Growth hormone family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDNA polymerase mu
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Synonyms gene name
- polymerase (DNA directed), mu
- polymerase (DNA) mu
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNA polymerases
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-galactosyltransferase 7
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Synonyms gene name
- xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 4-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNRAS proto-oncogene, GTPase
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Synonyms gene name
- neuroblastoma RAS viral (v-ras) oncogene homolog
- neuroblastoma RAS viral oncogene homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAS type GTPase family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesyndecan 3
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Synonyms gene name
- syndecan 3 (N-syndecan)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Syndecans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphatidylinositol glycan anchor biosynthesis class N
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Synonyms gene name
- phosphatidylinositol glycan, class N
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Phosphatidylinositol glycan anchor biosynthesis
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWASP like actin nucleation promoting factor
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Synonyms gene name
- Wiskott-Aldrich syndrome-like
- Wiskott-Aldrich syndrome like
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-10-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Wiskott-Aldrich Syndrome protein family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-02-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data