Mouse Monoclonal IgG1 Anti-Neurofilaments 160 kDa [RNF 403]

  • Catalog number
    NF103
  • Price
    Please ask
  • Size
    1 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Purified neurofilament proteins from calf brain
  • Specificity
    Neurofilaments 160 kDa
  • Species reactivity
    Human, cattle
  • Product presentation
    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
  • Product category
    Primary Antibody
  • Antibody s raised in
    Mouse
  • Antibody s isotype
    IgG1
  • Antibody s clone
    RNF 403
  • Product is supplied as
    liquid concentrate
  • Recommended concentration for use
    1:10 - 1:20
  • Protein concentration
    N/A
  • Positive control
    Nerve tissue
  • Pretreatment
    No protease pre-treatment required! Blocking of endogenous biotin and alkaline phosphatase is recommended, if avidin/biotin systems are used.
  • Tested applications
    IHC(P)
  • Recommended secondary reagent
    We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
  • Storage method
    Store at 2-8°C
  • UniProt number
    Go to UniProt/SwissProt
  • Litterature
    1. Osborn M., Altmannsberger M., Shaw G., Schauer A., Weber K. (1982) Various sympathetic derived human tumors differ in neurofilament expression. Use in Diagnosis of neuroblastoma, ganglioneuroblastoma and pheochromocytoma. Virchows Arch. Cell Pathol. 40; 141-156. 2. Alfonsi F., Darmon M., Forest N., and Paulin D. (1983) Intermediate sized filaments as markers of neuronal differentiation in the role of cell interaction in early neurogenesis. In: Plenum Publishing Corporation A.M. Duprat, A. Kato, and M. Weber eds. pp 157-176. 3. Osborn M., and Weber K. (1983) Biology of disease tumor diagnosis by intermediate filament typing. Lab. Invest. 48; 372-394. 4. Hacker GW, Polak JM, Springall DR, Ballesta J, Cadieux A, Gu J, Trojanowski JQ, Dahl D, Marangos PJ. (1985) Antibodies to neurofilament protein and other brain proteins reveal the innervation of peripheral organs. Immunochemistry, 82, 581-593. 5.. Osborn M., Dirk T., Käser H., Weber K., and Altmannsberger M. (1986) Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma. Am. J. Pathol. 122; 433-442.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The kilo Daltons subunit weight of Bioprime compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    TRIM31
  • Short name
    Bioprime
  • Technique
    Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Isotype
    IgG1
  • Species
    Mouse, Mouses
  • Alternative name
    Neurofilaments 160 kDa
  • Alternative technique
    murine, antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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