MCM2 Antibody

• Catalog number
  3758-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  MCM2
• Target Alternative Name
  Mitotin; BM28, Nuclear protein BM28; cdc19; CCNL1, cyclin like-1; CDCL1, CDC like-1; D3S3194; KIAA0030
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse
• Immunogen Sequence
  Synthetic peptide of human MCM2
• Accession
  NP_0045117.2
• Gene Id
  4171
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation
• Application And Usages
  Western blotting (1:500 – 1:2500) and Immunoprecipitation. HeLa cell extract can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MCM2 of human origin. Reactivity to mouse is expected due to sequence homology. Reactivity to other species has not been tested.
• Description
  The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-©-induced Stat1å transcription activation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MCM2  
• Gene symbol
  MCM7, MCM2
• Short name
  MCM2 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  MCM2 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6950
• Gene
  MCM7
• Long gene name
  minichromosome maintenance complex component 7
• Synonyms gene
  • MCM2
• Synonyms gene name
  • minichromosome maintenance deficient (S. cerevisiae) 7
  • MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
• Synonyms
  • CDC47
  • PPP1R104
• Synonyms name
  • protein phosphatase 1, regulatory subunit 104
• Locus
  7q22.1
• Discovery year
  1994-12-13
• Entrez gene record
  4176
• Pubmed identfication
  • 7842741
• Classification
  • MCM family
  • MicroRNA protein coding host genes
  • minichromosome maintenance 2-7 complex
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000154671

Gene info

• Identity
  HGNC:6944
• Gene
  MCM2
• Long gene name
  minichromosome maintenance complex component 2
• Synonyms gene
  • CCNL1
  • CDCL1
• Synonyms gene name
  • minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
  • MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• Synonyms
  • D3S3194
  • KIAA0030
  • BM28
  • cdc19
  • DFNA70
• Synonyms name
  • mitotin
• GenBank acession
  • X67334
• Locus
  3q21.3
• Discovery year
  1993-09-30
• Entrez gene record
  4171
• Pubmed identfication
  • 1710453
  • 8258304
  • 26196677
• Classification
  • MCM family
  • minichromosome maintenance 2-7 complex
• VEGA ID
  OTTHUMG00000159637

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products