Lipoxygenase Activity Assay Kit (Fluorometric)
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Catalog number
K978-100
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Price
Please ask
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Size
100 assays
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Description
Highly Sensitive Assay
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Summary
• Detection method- Fluorescence (Ex/Em 500/536 nm) • Species reactivity- Mammalian • Applications-LOX Activity Assay is highly sensitive with detecting limit approximately 0.004 mU/ mg
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Detection Method
Fluorescence (Ex/Em 500/536 nm)
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Species Reactivity
Mammalian
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Applications
Measurement of LOX activity in various tissues/cell extracts, biological fluids, and purified proteins - Determination of LOX activity associated with pathological conditions -Characterization of activity of purified LOX enzymes
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Sample Type
Cell/tissue lysates • Biological Fluids
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Features Benefits
• Simple procedure; takes only ~40 min • Fast and convenient
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Lipoxygenases are non heme iron - containing dioxygenases. These enzymes convert unsaturated fatty acids to epoxides; for example the synthesis of leukotrienes from arachidonic acid. They play an important role in cell proliferation, differentiation and inflammation; and have been implicated in inflammation and hyper proliferation mediated diseases like asthma, rheumatoid arthritis and cancer. In BioVision’s Lipoxygenase (LOX) activity assay, lipoxygenase converts the LOX substrate to an intermediate that reacts with the probe generating a fluorescent product. The increase in fluorescent signal can be recorded at Ex/Em 500/536 nm and is directly proportional to LOX activity. The kit includes 5-lipoxygenase enzyme (LOX Enzyme) as a positive control. A lipoxygenase inhibitor that completely inhibits lipoxygenase activity is also included in order to calculate the specific activity of LOX in biological samples. The kit can detect as low as 0.004 mU/ mg protein.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Short name
Lipoxygenase Activity Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
Lipoxygenase Activity test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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