IgG Isotype Control Fc fusion protein FITC

  • Catalog number
    35R-0013
  • Price
    Please ask
  • Size
    50 µg
  • Category
    Biological Reagents
  • Antibody Subtype
    Isotype Controls
  • Area of research
    Immunology
  • Raised in
    Hamster
  • Isotypes
    IgG
  • Concentration
    500 ug/ml
  • Form Buffer
    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
  • Storage
    Store at 2-8 deg C. Do not freeze. Protect from light.
  • Shipping conditions
    Blue Ice
  • Tested for
    FC
  • Usage Recommendations
    FC: ≤ 1 ug/test
  • Assay Information
    A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Additional Information
    This antibody is offered in 2 formats.
  • URL
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
  • Properties
    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Group
    isotypes
  • Gene target
    protein  
  • Gene symbol
    FCGBP, FCRL2
  • Short name
    IgG Isotype Control Fc protein FITC
  • Technique
    Control, Isotype, FITC, IgG, FC, controls, Fluorescein, IgGs, Isotypes and controls
  • Isotype
    IgG, IgG
  • Label
    FITC
  • Alternative name
    Syrian Hamster monoclonal IgG Isotype Control Fc fusion protein (FITC)
  • Alternative technique
    control, controls, fluorescine, immunoglobulins
  • Tissue
    control
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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