IgG Isotype Control antibody biotin

  • Catalog number
    61R-1586
  • Price
    Please ask
  • Size
    50 µg
  • Category
    Biological Reagents
  • Antibody Subtype
    Isotype Controls
  • Area of research
    Immunology
  • Raised in
    Hamster
  • Specificity
    Human
  • Isotypes
    IgG
  • Clone
    299Arm
  • Conjugate Tag
    Biotin
  • Concentration
    500 ug/ml
  • Form Buffer
    Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
  • Storage
    Store at 2-8 deg C. Do not freeze. Protect from light.
  • Shipping conditions
    Blue Ice
  • Tested for
    FC
  • Usage Recommendations
    FC: ≤ 0.25 ug/test
  • Assay Information
    A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • URL
  • Properties
    If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Conjugation
    Biotinylated
  • Description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Group
    isotypes
  • French translation
    anticorps
  • Short name
    IgG Isotype Control antibody
  • Technique
    Antibody, Control, Isotype, IgG, antibodies against human proteins, antibodies for, controls, IgGs, Isotypes and controls
  • Isotype
    IgG, IgG
  • Label
    Biotin
  • Alternative name
    Armenian Hamster monoclonal IgG Isotype Control antibody (biotin)
  • Alternative technique
    antibodies, control, controls, immunoglobulins
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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