Anti-5-Hydroxymethylcytosine Antibody

• Catalog number
  A1295-50
• Price
  Please ask
• Size
  50 µg

• Antibody Target
  5-hmc
• Target Alternative Name
  5-hmc, 5hmc
• Antibody Type
  Monoclonal
• Clone
  4D9
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  Phosphate Buffer 10mM - NaCl 0.15 M - pH 7.5
• Purification
  Affinity purified
• Application
  ELISA, hMeDIP IF, Dot Blot
• Positive Control
  Dot Blot, ELISA
• Application And Usages
  ELISA: 1:1000, hMeDIP: 1-3 µg per IP, IF: 1:500, Dot Blotting: 1:2,000
• Description
  5-hydroxymethylcytosine (5-hmC) is a modified base form of cytosine recently found in human/mouse brain and in embryonic stem cells. This DNA pyrimidine nitrogen base can be generated by oxidation of 5-methylcytosine, a reaction mediated by the ten-eleven translocation (TET) family of the 5-mC hydroxylases. The function of this base is still not elucidated but it is believed to play an important role in switching genes on and off.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  5-Hydroxymethylcytosine  
• Gene symbol
  HMCES
• Short name
  Anti-5-Hydroxymethylcytosine Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG1
• Alternative name
  Antibody to5-Hydroxymethylcytosine (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:24446
• Gene
  HMCES
• Long gene name
  5-hydroxymethylcytosine binding, ES cell specific
• Synonyms gene
  • C3orf37
• Synonyms gene name
  • chromosome 3 open reading frame 37
  • 5-hydroxymethylcytosine (hmC) binding, ES cell-specific
• Synonyms
  • DC12
  • SRAPD1
• Synonyms name
  • SOS response associated peptidase domain containing 1
  • embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein
• GenBank acession
  • AF201934
• Locus
  3q21.3
• Discovery year
  2006-01-02
• Entrez gene record
  56941
• Pubmed identfication
  • 23434322
  • 23945014
• RefSeq identity
  • NM_020187
• VEGA ID
  OTTHUMG00000159452

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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