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Bulletblenders sonication use
Equipment
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The use of this ultrasonic sonicator
Pressure Cell
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Weight of the sonicator or reagent
2.5
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Harmonized code of the instrument
9027.80.8000
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Nature of the sonication instrument or reagent for cell lyzis
Capillary loader for packing LC/MS columns and loading MS. 8500 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules
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Unit of homogenizing sonnicator sold
each
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Technical cell or tissue disruption
Homogenisation and disruption can be achieved by the mechanical Bullet blenders in a simular way but with less heating as with sonicators for cellular disruption and lysis. Instread of using sonication with utrasonic disruption of cells or tissues, the bullet blender technique and bullet blender gold will use gentle and heat absent cell lysis. Bullet Blenders can use more than 50gr of cell quantities. Homogenisers of French press for e.Coli lysation are less performant than bullet blenders even with 2 passages at 15,000 psi! DNAse can be added to preserve DNA for degradation for Epigenetics applications or for PCR.
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Description
For cells, cell lines and tissues in culture till half confluency.
