This antibody has been succesfully tested for use in following methods: Western blot, IHC.
Target of this antibody: Nuclerostemin
Formulation of this antibody: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative names of antibody target: GNL3; E2IG3; NS; Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47; Nucleolar GTP-binding protein 3; Nucleostemin
Recommended usage: WB: 1:1000, IHC: 1:10 – 1:50.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human.
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Immunogene sequence: This Nucleostemin (GNL3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-487 amino acids from the C-terminal region of human Nucleostemin (GNL3).
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: Nucleostemin, also designated Nucleolar GTP-binding protein 3, is a member of the MMR1/HSR1 GTP-binding protein family. It is expressed in the nucleoli of adult CNS stem cells, primitive bone marrow cells, and embryonic stem cells and in several cancer cell lines. Nucleostemin is often used as a stem cell marker. Overexpression or depletion of the protein can reduce cell proliferation in CNS stem cells. Nucleostemin shuttles between the nucleus and the nucleolus and may be important in maintaining the proliferative capacity of stem cells. Nucleostemin is important in the growth regulation of liver cancer, gastric cancer and several other cancer types. The gene encoding Nucleostemin is localized to chromosome 3p21.1.
Form of antibody: Liquid
Concentration of antibody: N/A
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps