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Immunogen
His-tagged and purified PfHSP70, C-terminus (AA 365-681)
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Antibody s target
P. falciparum HSP70
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Antibody s full description
Rabbit Anti-P. falciparum HSP70 (P. falciparum) Polyclonal
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Primary research fields
Cancer, Heat Shock
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
HSP70_PLAFA Antibody, Cytoplasmic antigen 74.3 kDa protein Antibody
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Verified applications
WB, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Plasmodium falciparum
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Antibody s recommended dilutions for use
WB (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~ 70kDa. Specific to P. falciparum and does not cross-react to any protein from Human erythrocytes.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
0.15 µg/ml of SPC-186 was sufficient for detection of PfHSP70 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. PfHSP70-I (PF08_0054) is the major cytosolic HSP70 in Plasmodium falciparum. It is abundantly expressed in the blood stages of the parasite and is thought to constitute 1-2% of total parasite protein. It is induced upon heat shock. It is present in the parasite in different complexes with PfHSP90 and some PfHSP40 (6, 7). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at more.
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Bibliography
1. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1) 1-17. 2. Rothman J. (1989) Cell 59: 591 -601. 3. DeLuca-Flaherty, et al. (1990) Cell. 62: 875-887. 4. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 5. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 6. Pesce E.R., et al. (2008) Int J Biochem Cell Biol. 40(12): 2914-26. 7. Pavithra S.R, Banumathy G., Joy O., Singh V., Tatu U. (2004) J Biol Chem. 279(45): 46692-9.
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Released date
27/Apr/2009
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NCBI number
M19753
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Gene number
Refer to GenBank
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Protein number
P11144
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PubMed number
26147206|25751816|23615908
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Tested applications
Immunocytochemistry/Immunofluorescence|Western Blot|Immunocytochemistry/Immunofluorescence
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Tested reactivity
P. falciparum|P. falciparum|P. falciparum
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Parasite Lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (SPC-186). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (SPC-186) at 1:2000. Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Parasite Lysates (SPC-186)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
HSPA1B, HSPA14, HSPA1A, HSPA1L
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Short name
Rabbit Anti-P. falciparum HSP70 (P. falciparum) Polyclonal: Streptavidin
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Technique
Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
Streptavidin
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Alternative name
production species: rabbit Antibody toP. falciparum HSP70 (P. falciparum) polyclonal: biotinilated-binding streptavidin
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Alternative technique
polyclonals, rabbit-anti, antibodies
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