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Immunogen
Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
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Antibody s target
Rat Aquaporin 3
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Antibody s full description
Rabbit Anti-Rat Aquaporin 3 Polyclonal
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Primary research fields
Neuroscience, Ion Channels, Aquaporins
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
AQP3 Antibody, AQP 3 Antibody, AQP-3 Antibody, Aqp3 Antibody, AQP3_HUMAN Antibody, Aquaglyceroporin-3 Antibody, Aquaporin 3 (GIL blood group) Antibody, Aquaporin 3 (Gill blood group) Antibody, Aquaporin-3 Antibody, Aquaporin3 Antibody, GIL Antibody, Gill blood group Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Protein A Purified
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Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~31.5kDa. May detect larger glycosylated bands ~35-50kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
0.5 µg/ml of SPC-504 was sufficient for detection of aquaporin 3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4).
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Bibliography
1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. Sasaki S., Ishibashi K., and Marumo F. (1998) Annu Rev Physiol. 60: 199-220. 4. Dibas A.I., Mia A.J., Yorio T. (1998) Proc Soc Exp Med. 219(3): 183-199.
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Released date
6/Dec/2011
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NCBI number
NP_113891.1
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Gene number
65133
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Protein number
P47862
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PubMed number
27413198
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Rabbit Anti-Aquaporin 3 Polyclonal Antibody (SPC-504). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody (SPC-504) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green). | Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 3 protein using Rabbit Anti-Aquaporin 3 Polyclonal Antibody (SPC-504). Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody (SPC-504) at 1:2000. Rabbit Anti-Aquaporin 3 Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-504) | Rabbit Anti-Aquaporin 3 Antibody used in Western blot (WB) on Rat kidney inner medullary homogenates (SPC-504)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
