HAT Activity Colorimetric Assay Kit

• Catalog number
  K332-100
• Price
  Please ask
• Size
  100 assays

• Description
  Non-radioactive assay for end point & kinetic studies
• Summary
  • Detection method- Absorbance (440 nm) • Species reactivity- Mammalian • Applications- Detects HAT activity by colorimetric method in mammalian samples
• Sample Type
  Cell and tissue lysates
• Features Benefits
  • Simple procedure • Fast and convenient • The detection can be continuous and suitable for kinetic studies
• Storage Conditions
  -20°C
• Shipping Conditions
  dry ice
• Shelf life
  12 months
• Background
  Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. BioVision’s HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  HAT   Activity   Kit  
• Gene symbol
  TMPRSS11D
• Short name
  HAT Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  HAT Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:24059
• Gene
  TMPRSS11D
• Long gene name
  transmembrane serine protease 11D
• Synonyms gene name
  • transmembrane protease, serine 11D
• Synonyms
  • HAT
  • ASP
• Synonyms name
  • airway trypsin like protease
  • adrenal secretory serine protease
• GenBank acession
  • AB002134
• Locus
  4q13.2
• Discovery year
  2005-03-11
• Entrez gene record
  9407
• Pubmed identfication
  • 9565616
  • 9070615
• RefSeq identity
  • NM_004262
• Classification
  • Type II transmembrane serine proteases
• VEGA ID
  OTTHUMG00000129300

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