PCAF Antibody

• Catalog number
  6675-100
• Price
  Please ask
• Size
  100 μg

• Antibody Target
  PCAF
• Target Alternative Name
  KAT2B; PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor.
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Recombinant human PCAF protein (Cat # 1137)
• Accession
  Q92831
• Gene Id
  8850
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.
• Purification
  Affinity Purified Antibody
• Application
  Western blot
• Positive Control
  Western Blot: Recombinant protein
• Application And Usages
  Western blot: 1-4 µg/ml.
• Description
  The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. P300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  PCAF  
• Gene symbol
  KAT2A, KAT2B
• Short name
  PCAF Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  PCAF (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4201
• Gene
  KAT2A
• Long gene name
  lysine acetyltransferase 2A
• Synonyms gene
  • GCN5L2
• Synonyms gene name
  • GCN5 general control of amino-acid synthesis 5-like 2 (yeast)
  • K(lysine) acetyltransferase 2A
• Synonyms
  • GCN5
  • PCAF-b
• GenBank acession
  • AF029777
• Locus
  17q21.2
• Discovery year
  1996-12-17
• Entrez gene record
  2648
• Pubmed identfication
  • 8552087
• RefSeq identity
  • NM_021078
• Classification
  • GCN5 related N-acetyltransferases
  • ATAC complex
  • SAGA complex
  • Lysine acetyltransferases
  • Bromodomain containing
• VEGA ID
  OTTHUMG00000133504

Gene info

• Identity
  HGNC:8638
• Gene
  KAT2B
• Long gene name
  lysine acetyltransferase 2B
• Synonyms gene
  • PCAF
• Synonyms gene name
  • p300/CBP-associated factor
  • K(lysine) acetyltransferase 2B
• Synonyms
  • P/CAF
  • GCN5
  • GCN5L
• GenBank acession
  • U57316
• Locus
  3p24.3
• Discovery year
  1999-02-10
• Entrez gene record
  8850
• Pubmed identfication
  • 8684459
  • 9722949
• RefSeq identity
  • NM_003884
• Classification
  • MicroRNA protein coding host genes
  • Bromodomain containing
  • GCN5 related N-acetyltransferases
  • ATAC complex
  • SAGA complex
  • Lysine acetyltransferases
• VEGA ID
  OTTHUMG00000130481

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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