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Antibody Target
CD9 (biotin conjugated)
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Target Alternative Name
DRAP-27 (Diphtheria Toxin Receptor-associated Protein)
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Antibody Type
Monoclonal
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Species Reactivities
Human
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Immunogen Sequence
Pre-B cell line NALM-6
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
1 mg/ml
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Formulation
1 mg/ml in phosphate buffered saline (PBS) with sodium azide (15 mM), Approx. pH: 7.4.
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Purification
Protein A affinity chromatography
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Application
WB, ELISA, IHC, IP
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Positive Control
WB, ELISA, IHC, IP
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Application And Usages
WB (1:1000), ELISA (1:500), IHC, IP
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Description
Anti-CD9 recognizes a human 24-kiloDalton (kDa) single-chain cell-surface glycoprotein (p24) belonging to the tetraspanin family which transverse the membrane four times. The CD9 antigen has a very broad tissue distribution. It is present on basophils, eosinophils, monocytes, pre-B cells, B cells, and various leukemic cell lines (erythroid, myeloid, some T-lymphoid, pre–B-lymphoid). It is also found on follicular center cells, sinus histocytes, macrophages, Kupffer cells, osteoclasts, hepatocytes, bile duct endothelium, renal glomeruli, proximal and distal tubuli, epithelia (intercellular spinous spaces) of skin and mucosa, fibroblasts, connective tissue, endothelium, smooth muscle, cardiac muscle, synovial lining cells, brain white matter, peripheral nerves and human keratin. In addition to being a co-stimulatory molecule, CD9 antigen activates platelets and induces mitogenesis and kinase activity and is involved in cell migration, cell adhesion, and integrin signaling.
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Storage Condition
4°C
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Shipping Condition
gel pack
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Shelf Life
Do not use after expiration date stamped on vial label.
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Biovision supplies antibodies that are for research of human proteins.
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Conjugation
Biotinylated
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French translation
anticorps
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Gene target
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Gene symbol
CD9
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Short name
Anti- CD9 conjugated antibody
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Technique
anti-Human, Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, antibody Conjugates
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Isotype
Mouse IgG1
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Label
Biotin
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Species
Human, Humans
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Alternative name
Antibody tohuman CD9 molecule biotinilated coupled (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
CD9 molecule, BTCC-1 and DRAP-27 and MIC3 and MRP-1 and TSPAN-29 and TSPAN29, CD9 and IDBG-13612 and ENSG00000010278 and 928, protein binding, Extracellular, Cd9 and IDBG-189938 and ENSMUSG00000030342 and 12527, CD9 and IDBG-640522 and ENSBTAG00000014764 and 280746
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