CD45RO FITC Monoclonal Antibody (Clone UCHL1)

  • Catalog number
    6960-100
  • Price
    Please ask
  • Size
    100 tests
  • Antibody Target
    CD45RO
  • Target Alternative Name
    CD45RO
  • Antibody Type
    Monoclonal
  • Clone
    UCHL1
  • Species Reactivities
    Human
  • Immunogen Sequence
    Human CD45RO
  • Accession
    P08575
  • Gene Id
    5788
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
  • Purification
    Affinity chromatography.
  • Application
    Flow cytometry
  • Positive Control
    FACS: Human peripheral blood lymphocytes
  • Application And Usages
    Flow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (0.5 µg).
  • Description
    CD45, a transmembrane multifunctional glycoprotein, is a member of Type I receptor-linked PTPase family and is expressed as multiple isoforms due to alternative splicing. Expression of these isoforms is highly regulated and shift in this expression determines T-cell activation. CD45RB consists of exon B and is predominantly expressed in naïve T-cells secreting IL-2. Its expression is low in primed/memory T cells, cells that express Th2 cytokines such as IL-4 and IL-10 and population of T-cells with regulatory function. Immunotherapy with CD45RB antibody is being widely studied in transplantation and vaccination. CD45 antibodies are commonly used to identify tumors of lymphoid origin. The UCHL1 monoclonal antibody specifically reacts with human CD45RO, a 180 kDa isoform of the leukocyte common antigen CD45. CD45RO is a transmembrane glycoprotein with tyrosine phosphatase activity and is expressed by majority of thymocytes, monocytes, granulocytes, and activated memory T lymphocytes. The subsets of peripheral T lymphocytes can be discriminated by using the CD45RO and CD45RA expressing cells.
  • Storage Condition
    4°C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    UCHL1-DT, UCHL1
  • Short name
    CD45RO FITC Monoclonal Antibody (Clone UCHL1)
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
  • Isotype
    Mouse IgG1, Kappa
  • Label
    FITC
  • Alternative name
    CD45RO fluorecein monoclonal (antibody to-) (clonality UCHL1)
  • Alternative technique
    antibodies, fluorescine
  • Clone name
    UCHL1
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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