Clonality Polyclonal Antibody
Other name Anti-MYL9(Ser20) Polyclonal
Host organism Rabbit (Oryctolagus cuniculus)
Background information Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Also known as MYL9(Ser20) Polyclonal Antibody
Swiss Prot P24844
Specificity This antibody reacts specifically with MYL9(Ser20)
Modification site Ser20
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Conjugated with ALEXA FLUOR® 594
Gene ID 10398
Clone Polyclonal Antibodies
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Synonyms LC20; MLC2; MRLC1; MYRL2; MLC-2C; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MYL9
Subcellular locations Intracellular
Concentration 1ug per 1ul
Purification method Purified by Protein A.
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Type Conjugated Primary Antibody
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Dog, Rabbit
Excitation emission 590nm/617nm
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Target Protein/Peptide MYL9 Ser20
Antigen Source KLH conjugated synthetic phosphopeptide derived from human Myosin Regulatory Light polypeptide 9 around the phosphorylation site of Ser20
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.