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Synonyms
FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Fc gamma RIIIa; IgG Fc receptor III-2
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Alternative_names
FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Fc gamma RIIIa; IgG Fc receptor III-2
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Description
Protein encoding the extracellular domain of human CD16a receptor
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Recombinant
1
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Source
HEK293 cells
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Purity by SDS PAGE
≥ 97% as determined by SDS-PAGE
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Biological activity
Activity of CD16a was measured by its binding ability in a functional ELISA. Immobilized human CD16a at a concentration of 10 ug/ml (100 ul/well) can bind biotinylated human IgG1.
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Molecular Weight
The recombinant human CD16a consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. The protein migrates as a band at ~45.2 kDa due to glycosylation, in SDS-PAGE under reducing conditions.
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Storage Temp
-20ºC
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween-80.
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Reconstitution Instructions
Reconstitute in sterile water to a concentration of 250 µg/ml by adding 200 µl water to 50 µg of protein.
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Background Information
CD16a/FCGR3A is a low affinity receptor for the Fc region of IgG. The two genes for human FCGR3A encode for CD16A and CD16B. CD16a is a transmembrane protein found on the surface of natural killer cells, activated monocytes and macrophages and a subset of T cells. CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a can cause susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
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Amino acid sequence
The DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) precursor was expressed with a C-terminal polyhistidine tag. The predicted N terminal is Gly 17.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
