This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Alternative names of antibody target:
Insulin A chain, Insulin B chain, Proinsulin
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Clonality of antibody:
E. coli expressed recombinant human Insulin
How to handle this antibody:
The antibody solution should be gently mixed before use.
Insulin is important in stimulating the uptake of glucose from blood in the skeletal muscle and adipose tissue. It is secrered by pancreatic β cells throμgh a glucose sensing pathway. Insulin also increases cell permeability to monosaccharides, amino acids and fatty acids. Insulin is first formed as a precursor molecule, preproinsulin which is then processed into proinsulin and finally to the mature insulin hormone. A mature form of insulin has an A chain and a B chain that are connnected by two dilsufide bonds. Defects in insulin are the cause of type-II diabetes mellitus.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
antibody to-Insulin (Antibody to)