Flag Tag antibody
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Catalog number10R-10268
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PricePlease ask
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Size100 µg
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CategoryPrimary Antibody
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Antibody SubtypeMonoclonal Antibodies
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Area of researchMiscellaneous
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Type of ImmunogenFlag Tag antibodies were raised in Mouse using a synthetic peptide (DYKDDDDK) coupled to KLH .
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Raised inMouse
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SpecificityFlag Tag antibody recognizes overexpressed proteins containing Flag epitope tag (Dtag) fused to either amino or carboxy-termini of targeted proteins in transfected mammalian cells
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Cross ReactivityMouse, Rat
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IsotypesIgG2b
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CloneM78633
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Concentration1 mg/ml
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Form BufferProvided in PBS buffer, pH 7.4 with 0.05% NaN3
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StorageStore at -20 deg C for 1 year, store at 2-8 deg C for up to 1 month
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Shipping conditionsBlue Ice
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Tested forWB; IP
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Usage RecommendationsWB: 1:1000-4000, IP: 1:200-400
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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ConjugationFlag
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French translationanticorps
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Gene target
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Gene symbolTRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
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Short nameTag antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for, Tags
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LabelFlag
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Alternative nameMouse monoclonal Flag Tag antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 15 (anticodon UAG)
- transfer RNA-Leu (TAG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 7 (anticodon UAG)
- transfer RNA-Leu (TAG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 4-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 40 (anticodon UAG) pseudogene
- transfer RNA-Leu (TAG) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data