Goat anti Mouse IgD (Fc specific)

  • Catalog number
    GAM/IgD(Fc)
  • Price
    Please ask
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is restricted to the Fc portion of the IgD molecule, which represents strict isotype (class specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum belonging to different allotypic groups, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with the purified IgD from Balb/C and C57Bl. No precipitation reaction is obtained with purified IgG, including all subclasses, IgA, IgM, IgG/Fab fragments or other serum proteins. The antiserum also reacts with membranebound IgD in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgD in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgD from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
  • Antibody come from
    Highly purified polyclonal and monoclonal IgD isolated from Balb/C and C57Bl serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
  • Clone
    Polyclonal
  • Antigen antibody binding interaction
    Goat anti Mouse IgD (Fc specific) Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Mouse
  • Antibody s reacts with these species
    The antiSerum does not cross-react with any other component of the Mouse Ig system. Inter-species cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants, but this has not been tested because of the very low level of IgD in most species.
  • Antibody s specificity
    Precipitating polyclonal Goat antiSerum to Mouse IgD, Fc specific
  • Research interest
    Veterinary
  • Application
    Immunoprecipitation
  • Antibody s suited for
    Immunoprecipitation. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Precipitin titre 1:32 when tested against monoclonal IgD containing mouse serum in agarblock immunodiffusion titration.
  • Storage
    Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Latin name
    Capra aegagrus hircus, Mus musculus
  • Properties
    Immunoglobulin D (IgD) is an antibody isotype that makes up about 1% of proteins in the plasma membranes of mature B-lymphocytes where it is usually coexpressed with another cell surface antibody called IgM. IgD is also produced in a secreted form that is found in very small amounts in blood serum, representing 0.25% of immunoglobulins in serum for virus detection. Anti-viral immunoglobulin D antibodies have a diagnostic value.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Gene target
  • Short name
    Goat anti Mouse IgD (Fc specific)
  • Technique
    anti mouse, Mouse, anti, Goat, IgD, FC, antibody to, anti mouse antibodies, goats, IgDs, mouses
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    not specified, IgD
  • Label
    unlabeled
  • Species
    Mouse, Goats, Mouses
  • Alternative name
    caprine antibody to Mouse IgD (fragment c specific)
  • Alternative technique
    murine, antibodies, caprine, immunoglobulin-d
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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