Mouse anti Cytokeratin 10 / Keratin K10
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Catalog numberMUB0320P
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PricePlease ask
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Size0,1 mg
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CategoryPrimary Antibodies
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Long descriptionCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual Human Cytokeratins are numbered 1 to 20. The various epithelia in the Human body usually express Cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Antibody come fromDE-K10 is a Mouse monoclonal IgG1, κ antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 Mouse immunized with a cytoskeletal preparation extracted from Human epidermis.
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Other descriptionEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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CloneDE-K10
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Antigen antibody binding interactionMouse anti Cytokeratin 10 / Keratin K10 Antibody
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Antibody is raised inMouse
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Antibody s reacts withCanine,Feline,Human
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityDE-K10 reacts exclusively with Cytokeratin 10 which is present in Keratinizing stRatified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
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Research interestCytoskeleton
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ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
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Antibody s suited forDE-K10 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
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StorageStore at 4°C, or in small aliquots at -20°C.
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Relevant referencesIvanyi, D., Ansink, A., Groeneveld, E., Hageman, P. C., Mooi, W. J., and Heintz, A. P. (1989). New monoclonal antibodies recognizing epidermal differentiation- associated Keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva, J Pathol 159, 7-12. Ivanyi, D., Ansink, A., Mooi, W. J., de Kraker, N. W., and Heintz, A. P. (1989). Absence of differentiation-related expression of Keratin 10 in early stages of vulvar squamous carcinoma, Differentiation 42, 124-9. Ivanyi, D., Groeneveld, E., Van Doornewaard, G., Mooi, W. J., and Hageman, P. C. (1990). Keratin subtypes in carcinomas of the uterine cervix: impliCations for histogenesis and differential diagnosis, Cancer Res 50, 5143-52. Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline Cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14. Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with Keratin monoclonal antibodies in canine tissues: urogenital tract, respiRatory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40. Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., van Doornewaard, G., and Misdorp, W. (1993). Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095-102. Vos, J. H., van den Ingh, T. S., Ramaekers, F. C., Molenbeek, R. F., de Neijs, M., van Mil, F. N., and Ivanyi, D. (1993). The expression of Keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region, Vet Pathol 30, 352-61 van Bommel, P. F., Kenemans, P., Helmerhorst, T. J., Gallee, M. P., and Ivanyi, D. (1994). Expression of Cytokeratin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix, Cancer 74, 2314-20. Stout, G.J., Westdijk, D., Calkhoven, D.M., Pijper, O., Backendorf, C.M.P., Willemze, R., Mullenders, L.H.F. and de Gruijl, F.R. (2005). Epidermal transit of repliCation-arrested undifferentiated Keratinocytes in UV-exposed XPC mice: an alternative to in situ apoptosis PNAS 102, 18980-85.
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Protein numberP13645
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolKRT10
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Short nameMouse anti Cytokeratin 10 / Keratin K10
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TechniqueMouse, anti, antibody to, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to Cytokeratin 10 / Keratin K10
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namekeratin 10
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Synonyms gene
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Synonyms gene name
- keratosis palmaris et plantaris
- keratin 10, type I
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-08-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Keratins, type I
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data