Human Recombinant Fumarase
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Catalog number
6384-100
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Price
Please ask
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Size
100 ug
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Synonyms
Human Recombinant Fumarase
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Alternative_names
Fumarate hydratase, FH, HLRCC, LRCC, MCL, MCUL1.
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Description
Involved in the TCA or Krebs cycle
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Recombinant
Yes
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Source
E. Coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per 1 ug of protein (determined by LAL method)
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Biological activity
Specific activity is > 1.0 unit/mg
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Results
>1.0 unit/mg
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Unit Definition
One unit is defined as the amount of enzyme that cleaves 1 µmole of L-Malate to Fumarate per minute at pH 7.5 at 25°C.
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Molecular Weight
50.2 kDa (467 aa, 44-510 aa)
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
12 months
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Concentration
1 mg/ml
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Appearance
Liquid
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Physical form description
1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0).
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Background Information
Fumarase (Fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-terminal extended mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor.
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Amino acid sequence
MASQNSFRIE YDTFGELKVP NDKYYGAQTV RSTMNFKIGG VTERMPTPVI KAFGILKRAA AEVNQDYGLD PKIANAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGELGSKI PVHPNDHVNK SQSSNDTFPT AMHIAAAIEV HEVLLPGLQK LHDALDAKSK EFAQIIKIGR THTQDAVPLT LGQEFSGYVQ QVKYAMTRIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GELILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK ETAIELGYLT AEQFDEWVKP KDMLGPK
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
FH
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Short name
Recombinant Fumarase
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
H. sapiens Rec. Fumarase
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
fumarate hydratase
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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