Medium 199 With Earle's salts and 25mM HEPES buffer Without L-Glutamine and Sodium bicarbonate

  • Catalog number
    AT094A-5X20L
  • Price
    Please ask
  • Size
    5X20L
  • Description
    Medium 199, With Earle's salts and 25mM HEPES buffer Without L-Glutamine and Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It is a complex medium That was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. When supplemented with serum, It can be used for growth of a wide variety of cells. Presently, It is used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.
  • Shelf life
    Use before expiry date given on the product label.
  • Storage
    Store at 2-8°C away from bright light
  • Appearance
    Off-white to Creamish white, homogenous powder.
  • PH without Sodium Bicarbonate
    4.80 -5.40
  • PH with Sodium Bicarbonate
    6.60 -7.20
  • Osmolality without Sodium Bicarbonate
    265.00 -305.00
  • Osmolality with Sodium Bicarbonate
    310.00 -350.00
  • Cultural Response
    The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content
    NMT 5EU/ml
  • Solubility
    Clear solution at 15.5gms/L
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    Acute Effects : May be harmful if swallowed. Exposure can cause : Stomach pains, vomiting, diarrhoea. Prolonged or repeated exposure may cause allergic reactions in certain sensitive individuals. Prolonged or repeated exposure may cause allergic reactions in certain sensitive individuals.
  • Ordering
    To order Medium 199 With Earle's salts and 25mM HEPES buffer Without L-Glutamine and Sodium bicarbonate, please use the Cat. Nr.AT094A-5X20L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Medium 199 With Earle's salts and 25mM HEPES buffer Without L-Glutamine and Sodium bicarbonate
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Group
    media
  • Gene target
  • Short name
    Medium 199 With Earle's salts 25mM Without L-Glutamine Sodium bicarbonate
  • Technique
    Medium, cell culture Media
  • Alternative name
    Medium 199 including Earle's salts and 25mM HEPES solution Without L-Glutamine and Sodium bicarbonate
  • Alternative technique
    cellculture
  • Virus
    hhv
MeSH Data
  • Name
  • Concept
    Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
  • Tree numbers
    • E05.481
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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